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Chemistry

Theses, Dissertations and Capstones

Theses/Dissertations

2019

Articles 1 - 6 of 6

Full-Text Articles in Physical Sciences and Mathematics

Organizing 6, 5-Carbon Nanotubes On Dna Origami Arrays, Kathryn A. Pitton Jan 2019

Organizing 6, 5-Carbon Nanotubes On Dna Origami Arrays, Kathryn A. Pitton

Theses, Dissertations and Capstones

Carbon nanotubes1 are cylindrical carbon molecules and can be considered to be composed of one atom thick sheets of carbon called graphene. They have been at the forefront of nanochemistry due to their strength and multiple properties such as absorption, fluorescence, semiconduction, and light scattering capability. DNA Origami (DO)2 has emerged as a novel domain for molecular design, resulting in the generation of numerous one, two, and three-dimensional structures that have provided platforms for the organization of organic species and inorganic nanoparticles through simple modifications. The use of DNA Origami to control the placement of single walled carbon …

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Synthesis Of 1,3-Di(4-Pyridinyl)Acetone, Zachary Tyler Boggs Jan 2019

Synthesis Of 1,3-Di(4-Pyridinyl)Acetone, Zachary Tyler Boggs

Theses, Dissertations and Capstones

Although isoelectronic to benzene, the reactivity of pyridine-derived compounds can be quite different. Notably, routes to prepare pyridyl analogs to known phenyl compounds can be very different from how the phenyl compounds are made, even when the nitrogen atom would appear to play an insignificant role in the reaction. This is the case for 1,3-di-4-pyridylacetone. Its phenyl analog has been known for over 90 years and is easily prepared, while the only reported synthesis of 1,3-di-4-pyridylacetone occurs in very low yield. We report a preliminary synthesis from common starting materials, albeit in low yield, and offer an explanation for why …

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Identifying C-Mannosylatedproteins In Raw264.7 Cells Via In Vitro Hsc70 Co-Immunoprecipitation Strategies, Nicholas Ryan Kegley Jan 2019

Identifying C-Mannosylatedproteins In Raw264.7 Cells Via In Vitro Hsc70 Co-Immunoprecipitation Strategies, Nicholas Ryan Kegley

Theses, Dissertations and Capstones

C-mannosylation is a specific type of glycosylation in which α-mannose is covalently bound to the C2 atom of a tryptophan via a carbon-carbon bond. Presently, it is known to occur on proteins that contain the W-x-x-W-x-x-W/C motif found within the Thrombospondin Type-1 Repeat (TSR), with ‘x’ denoting any amino acid. C-mannosylation has been implicated in a number of cellular functions, including protein secretion and type I cytokine receptor function. Previous research has shown that exogenous C-mannosylated peptides derived from TSR-containing proteins upregulate the production of the pro-inflammatory cytokine Tumor Necrosis Factor alpha (TNF-α) in RAW264.7 macrophage cells. This upregulation is …

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Characterizing The Effects Of Environmental Stressors On The Photosynthetic Capacity Of Chlorella Vulgaris, Amanda Louise Smythers Jan 2019

Characterizing The Effects Of Environmental Stressors On The Photosynthetic Capacity Of Chlorella Vulgaris, Amanda Louise Smythers

Theses, Dissertations and Capstones

Chlorella vulgaris is a unicellular green algae grown throughout the world. Due to its multiple trophic modes as well as its ability to maintain high rates of growth under adverse conditions, it has been of global interest for use in ecological contamination studies, biofuel feedstock optimization, and studies of photosynthetic electron transfer. Using a wide-range of methods for physiological and photosynthetic characterization, the studies within seek to further extend the usefulness of C. vulgaris in a variety of environmentally important studies. Once the protocols were optimized specifically for this alga, they could be applied in both ecologically relevant and biodiesel …

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Isolation And Identification Of Hsc70 Conjugates In Raw264.7 Murine Macrophage-Like Cells, Michael Arland Parsons Jan 2019

Isolation And Identification Of Hsc70 Conjugates In Raw264.7 Murine Macrophage-Like Cells, Michael Arland Parsons

Theses, Dissertations and Capstones

IL12R- β2 is a Type I cytokine receptor and contains the WXXW (WSNWS) sequence that often predicates the post-translational addition of mannose to a tryptophan residue via a carbon-carbon bond. This study will stimulate expression of IL12R- β2 in RAW 264.7 Macrophage-Like Cells by shutting down the extracellular signal kinase (ERK) pathway and introducing inflammatory agents lipopolysaccharide and CpG DNA in order to collect a concentrated sample of IL12R- β2. These samples will be analyzed for the presence of C-mannosyltryptophan residue

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Novel Dna Origami Based Lateral Flow Assay Development, Adrienne Walker Jan 2019

Novel Dna Origami Based Lateral Flow Assay Development, Adrienne Walker

Theses, Dissertations and Capstones

Lateral flow assays (LFA) are used for point-of-care qualitative diagnostics of an analyte of interest, often in non-laboratory environments. Traditionally, the format of a lateral flow assay is to utilize immobilized antibodies on a membrane as the capture probe in conjunction with a reporting immunological recognition system for an analyte captured between them in a sandwich format. However, there are several shortcomings of antibodies which recommend their replacement with other recognition elements, if possible. The research described in this thesis was directed toward using several of the inherent properties of DNA based Origami nanostructures to enable the construction of DNA …

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