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Expanding The Toolbox With Site-Specific Methods Of Bioconjugation, Tiauna S. Howard
Expanding The Toolbox With Site-Specific Methods Of Bioconjugation, Tiauna S. Howard
Seton Hall University Dissertations and Theses (ETDs)
Bioconjugation is an important tool for studying complex biological systems, with site-specificity being the major challenge. Reactions based on amino acid derived organocatalysts have been widely used in organic synthesis, particularly for the asymmetric synthesis of small molecules but this concept has not been vastly explored on biomolecules. To combat these limitations, two chemical strategies are developed to effectively attach synthetic molecules site specifically to proteins. First, a protein modification technique based on conjugation at a non-native functional handle, an aldehyde or ketone, is developed. This functional handle is chemically introduced onto the biomolecules before undergoing the organocatalyzed aldol reaction, …