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Development Of An Upper Level Undergraduate Research Based Laboratory: The Purification, Analysis, And Dinetic Study Of Cytochrome B5, Mary Mcphail
Senior Honors Theses and Projects
The purpose of this project was to develop an upper level undergraduate biochemistry laboratory (CHEM 453) based upon a novel research project, designed to replace an existing instructional laboratory that employs ‘cookbook’ exercises. Methods for the expression, purification, spectral analysis of structural integrity, and electron transfer capability of rabbit cyt b5 were adapted from published work done by Dr. Lucy Waskell of the University of Michigan and Dr. Mansuy of the University of Paris. In our hands, rabbit liver cyt b5 expressed in E. coli was purified to 45% specific content of the theoretical content. Additionally, 37 nmol …