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Chemistry

Senior Honors Theses and Projects

Theses/Dissertations

Autophagosome

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Understanding The Role Of Atg7 And Atg14 In Autophagy, Ronith Chakraborty Jan 2019

Understanding The Role Of Atg7 And Atg14 In Autophagy, Ronith Chakraborty

Senior Honors Theses and Projects

Autophagy is the process by which cytosolic components are trafficked to and degraded by the vacuole or lysosome. It plays a critical role in cellular health, aging, cancer, and neurodegenerative diseases. Atg7 and Atgl4 are enzymes required for the autophagic process in Saccharomyces cerevisiae. In this study, we hypothesized that Atg7 controls the size while Atg 14 controls the number of autophagosomes. Using western blotting, Pho8D.60 assay and transmission electron microscopy analysis, we found that Atg7 affects both the size and number of autophagosomes. In addition, we have created cells expressing various levels of Atgl4 using non-native promoters. In addition, …