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Full-Text Articles in Physical Sciences and Mathematics
An Investigation Of The Nature Of Plasminogen Activator Inhibitor Type-I (Pai-I) To Improve Upon Known Small Molecule Inhibitors, Justin A. Powers
An Investigation Of The Nature Of Plasminogen Activator Inhibitor Type-I (Pai-I) To Improve Upon Known Small Molecule Inhibitors, Justin A. Powers
Senior Honors Theses and Projects
Plasminogen activator inhibitor type-I (PAI-I ) is a serpin superfamily member protein that acts as the major inhibitor of tissue-type plasminogen activator (tPA) and urokinase-type plasminogen activator (uPA). At physiological levels, PAI-I plays an active role in regulating fibrinolysis, the process of normal blood clot degradation. Pathological levels of PAI-I have been linked to multiple health ailments: fibrosis, heart disease, cancer, and obesity. In recent years, PAI-1 has been the subject of targeted drug design. In this study, we identified a class of polyphenol compounds as the most capable of maintaining its inhibitory efficiency against vitronectin bound PAI-I in buffer …
Understanding The Role Of Atg7 And Atg14 In Autophagy, Ronith Chakraborty
Understanding The Role Of Atg7 And Atg14 In Autophagy, Ronith Chakraborty
Senior Honors Theses and Projects
Autophagy is the process by which cytosolic components are trafficked to and degraded by the vacuole or lysosome. It plays a critical role in cellular health, aging, cancer, and neurodegenerative diseases. Atg7 and Atgl4 are enzymes required for the autophagic process in Saccharomyces cerevisiae. In this study, we hypothesized that Atg7 controls the size while Atg 14 controls the number of autophagosomes. Using western blotting, Pho8D.60 assay and transmission electron microscopy analysis, we found that Atg7 affects both the size and number of autophagosomes. In addition, we have created cells expressing various levels of Atgl4 using non-native promoters. In addition, …
Characterization Of The Histone Binding Properties Of The Epigenetic Reader Protein Uhrf2 To H3 In Phd Domain, Marisa Gilliam
Characterization Of The Histone Binding Properties Of The Epigenetic Reader Protein Uhrf2 To H3 In Phd Domain, Marisa Gilliam
Senior Honors Theses and Projects
Every cell contains the same genetic code, to have cell differentiation this genetic code needs to be regulated on what is expressed. The regulation of gene expression without altering the genetic code itself is known as epigenetics. An example of epigenetic regulation can be seen between the binding of UHRF2 and H3 histones. The purpose of this project is to determine ifD363 in UHRF2 is important for histone H3 binding. To do so, D363 was mutated to alanine, lysine or asparagine. The mutant protein was expressed, purified, and its ability to binding to H3 was tested by fluorescence polarization. Compared …