Physical Sciences and Mathematics Commons^™

Using Ultra Performance Liquid Chromatography-Mass Spectrometric Based Metabolomics To Determine The Metabolic Differences Due To Low Temperature Or Genetic Mutation In Salmonella Enterica, Nicole Rhonda Mooney

Masters Theses

Metabolomics aims to identify and quantify all of the small molecules within a cell. The relative concentrations of metabolites within cells grown under different experimental conditions, or different species, can be compared using pool size data. Exposing cellular cultures to isotopically labeled compounds allows the progress of metabolites through metabolic pathways to be tracked, known as flux. In the first experiment, the metabolic effects of exposure to lower than optimal temperatures in wild type Salmonella enterica, i.e. cold shock, were determined. The metabolites involved in glycolysis, the citric acid cycle, and the pentose phosphate pathway had less efficient labeling …