Physical Sciences and Mathematics Commons^™

Carboxymethylethanolamine, A Biomarker Of Phospholipid Modification During The Maillard Reaction In Vivo, Jesus R. Requena, Mahtab U. Ahmed, C. Wesley Fountain, Thorsten P. Degenhardt, Sharanya Reddy, Cliff Perez, Timothy J. Lyons, Alicia J. Jenkins, John W. Baynes, Suzanne R. Thorpe

Faculty Publications

Nepsilon-(Carboxymethyl)lysine (CML) is a stable chemical modification of proteins formed from both carbohydrates and lipids during autoxidation reactions. We hypothesized that carboxymethyl lipids such as (carboxymethyl)phosphatidylethanolamine (carboxymethyl-PE) would also be formed in these reactions, and we therefore developed a gas chromatography-mass spectrometry assay for quantification of carboxymethylethanolamine (CME) following hydrolysis of phospholipids. In vitro, CME was formed during glycation of dioleoyl-PE under air and from linoleoylpalmitoyl-PE, but not from dioleoyl-PE, in the absence of glucose. In vivo, CME was detected in lipid extracts of red blood cell membranes, approximately 0.14 mmol of CME/mol of ethanolamine, from control and diabetic subjects, …