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Physical Sciences and Mathematics Commons

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Chemistry

Electronic Theses and Dissertations

2011

Bioimaging

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Self-Assembly And Photophysics Of Selected Organic Materials And Two-Photon Bioimaging With Profluorescent Nitroxides, Polyelectrolyte Nanoparticles, And Squaraine Probes, Hyo Yang Ahn Jan 2011

Self-Assembly And Photophysics Of Selected Organic Materials And Two-Photon Bioimaging With Profluorescent Nitroxides, Polyelectrolyte Nanoparticles, And Squaraine Probes, Hyo Yang Ahn

Electronic Theses and Dissertations

Two-photon absorption and upconverted fluorescence has been utilized in a variety of applications in pure science and engineering. Multiphoton-based techniques were used in this research in order to understand photophysical and chemical characteristics of several fluorescent dyes and to demonstrate some of their key applications. Two-photon fluorescence microscopy (2PFM) has become a powerful technique in bio-photonics for non-invasive imaging in the near-infrared (NIR) region (700~1000 nm) that often results in less photobleaching. In Chapter 1, there is a brief introduction to fluorescence, examples of fluorescence materials, and a discussion of the advantages of two-photon absorption. 2PFM imaging was utilized in …