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Full-Text Articles in Physical Sciences and Mathematics
Enzyme-Based Nitric Oxide Releasing Thin Films And Scaffolds, Mutha Merenna Nuwan Bhagya Gunasekera
Enzyme-Based Nitric Oxide Releasing Thin Films And Scaffolds, Mutha Merenna Nuwan Bhagya Gunasekera
ETD Archive
Nitric oxide synthase enzyme (NOS) embedded in thin films and scaffolds, when exposed to a solution of its substrate arginine, a source of reducing equivalents, and other required ingredients of the NOS reaction, can release fluxes of nitric oxide (NO). The latter is a molecule known to counteract platelet aggregation, and thus can prevent the thrombosis cascade on the surfaces of implantable medical devices. Therefore NO antithrombogenic regimens such as active coatings and embedded scaffolds have the potential to increase the lifespan of implantable biomaterials. Layer-by-layer electrostatic adsorption allows for assembly of multi-component protein/polyelectrolytes nanostructured films. Electrospun fiber matrices may …
Nos Oxygenase-Mediated Nitroalkane Catalytic Reduction: Impact On Nos Reaction, Praneeth Ivan Joel Fnu
Nos Oxygenase-Mediated Nitroalkane Catalytic Reduction: Impact On Nos Reaction, Praneeth Ivan Joel Fnu
ETD Archive
Organic nitroalkanes are used in many industries and for a variety of purposes. Their use is expanding and is steadily claiming new territories in our immediate environment. Some nitroalkanes, when they enter the human body, can be harmful as such or can be activated to generate reactive intermediates with known detrimental effects. P450 enzymes are known for metabolizing a variety of drugs and substances including organic nitroalkanes. Nitric oxide synthases (NOS) exhibit some similarities to P450 metalloenzymes and their potential interaction with nitroalkanes as xenobiotics is not well known. The present study investigates the effect of nitroalkanes on the catalytic …
Nos-Based Biopolymers: Towards Novel Thromboresistant No-Release Materials, Charbel Abou Diwan
Nos-Based Biopolymers: Towards Novel Thromboresistant No-Release Materials, Charbel Abou Diwan
ETD Archive
Nitric Oxide releasing biopolymers have the potential to prolong vascular graft and stent potency without adverse systemic vasodilation. It was reported in literature that eNOS-overexpressing endothelial cell seeding of synthetic small diameter vascular grafts decreased human platelet aggregation by 46 and bovine aortic smooth muscle cell proliferation by 67.2 in vitro. We hypothesized that incorporating the enzyme nitric oxide synthase (NOS) in biocompatible polymeric matrix will provide a source of NO that utilizes endogenous compounds to maintain an unlimited supply of NO. To test this hypothesis, we have incorporated the enzyme nitric oxide synthase into a polyethyleneimine film using a …
Hemeproteins Bathed In Ionic Liquids;Examining The Role Of Water And Protons In Redox Behavior And Catalytic Function, John Joseph Moran
Hemeproteins Bathed In Ionic Liquids;Examining The Role Of Water And Protons In Redox Behavior And Catalytic Function, John Joseph Moran
ETD Archive
We investigate the changing behaviors of myoglobin and nitric oxide synthase (NOS) in the near-absence of bulk water and/or protons by using ionic liquid butyl methyl imidazolium tetrafluoroborate as a non-aqueous milieu. Through direct charge transfer and metalloprotein-mediated catalytic reduction of oxygen and nitric oxide, we shed light on diverging aspects on how the two hemeproteins face the scarcity of water and/or protons in bulk. Isotopic effect investigations using D2O further elucidates kinetic aspects of proton transfer. Finally, in the case of NOS oxygenase, pterin cofactor binding and NOS-mediated catalytic oxidation of L-arginine in ionic liquids interrogates proton and water …
New Generation Of Electrochemical Sensors For Nitric Oxide;Ruthenium/Carbon-Based Nanostructures And Colloids As Electrocatalytic Platforms, W. Pubudu M. Peiris
New Generation Of Electrochemical Sensors For Nitric Oxide;Ruthenium/Carbon-Based Nanostructures And Colloids As Electrocatalytic Platforms, W. Pubudu M. Peiris
ETD Archive
Nitric oxide (NO) is an important intercellular messenger that acts in many tissues to regulate a diverse range of physiological and pathological processes. The physiologically implications of NO function are far from being completely understood. The multifaceted reactivity of NO prompted the need for accurate determination of the concentration of this molecule. However, it is difficult to detect nitric oxide, particularly in biological media and near live cells due to its short half-life, a result of its reactivity and the low levels of NO produced in vivo. As a result, the accurate and reliable detection of NO under varying experimental …
Study Of 3'-Untranslated Region Of Inducible Nitric Oxide Synthase And Identification Of Other Targets Of Gaitpathway, Sirisha Vadlamani
Study Of 3'-Untranslated Region Of Inducible Nitric Oxide Synthase And Identification Of Other Targets Of Gaitpathway, Sirisha Vadlamani
ETD Archive
Macrophages and other leukocytes play a vital role during inflammation. IFN-Îđ which is secreted by Th1 cells activates monocyte-macrophages and this activation leads to induction as well as suppression of certain genes expressed during inflammation. This suppression of genes may be potentially vital for resolution of inflammation. Upon IFN-Îđ stimulation, a Gamma-activated inhibitor of translation complex (GAIT) is formed, which binds to 3'-UTR of target mRNAs thereby silencing their translation. VEGF-A and ceruloplasmin are two proteins whose translation is suppressed by this pathway. The GAIT complex proteins have been identified as ribosomal protein L13a, glutamyl-prolyl tRNA synthatase (EPRS), NS-1 associated …