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Full-Text Articles in Physical Sciences and Mathematics
Cofactor Control Of A Vital Enzymatic Reaction;The Effect Of Factor Va On Thrombin Formation During Blood Coagulation, Jamila Hirbawi
Cofactor Control Of A Vital Enzymatic Reaction;The Effect Of Factor Va On Thrombin Formation During Blood Coagulation, Jamila Hirbawi
ETD Archive
The LONG-TERM goal of our research is to study and analyze the structure and function of the factor V molecule in order to understand its regulatory effects on the natural process of hemostasis and its role in the life-threatening development of deep venous thrombosis. The SHORT-TERM goal of our research is to identify the amino acid residue(s) of factor V that interact with prothrombin during the assembly and function of the prothrombinase complex in order to fully understand its particular role in maintaining the integrity of the blood coagulation cascade. The final goal of the coagulation cascade is the formation …
Factor Va Directs Catalysis By Factor Xa During Prothrombin Activation, Michael Anthony Bukys
Factor Va Directs Catalysis By Factor Xa During Prothrombin Activation, Michael Anthony Bukys
ETD Archive
Hemostasis occurs through the controlled activation and inactivation of clotting factors resulting in the arrest of bleeding without blockage to the vasculature, while thrombosis is the undesired formation of vascular blood clots, which adversely affects millions of people annually. Clotting factors circulate in blood as inactive zymogens, which are proteolytically activated in response to vascular injury, assembled into enzymatic complexes and in turn activate additional coagulation factors culminating in the production of thrombin from the enzymatic complex prothrombinase. The prothrombinase complex is composed of the activated enzymatic component factor Xa (fXa) complexed to the activated cofactor portion factor Va (fVa) …
Modeling The Human Prothrombinase Complex Components, Tivadar Orban
Modeling The Human Prothrombinase Complex Components, Tivadar Orban
ETD Archive
Thrombin generation is the culminating stage of the blood coagulation process. Thrombin is obtained from prothrombin (the substrate) in a reaction catalyzed by the prothrombinase complex (the enzyme). The prothrombinase complex is composed of factor Xa (the enzyme), factor Va (the cofactor) associated in the presence of calcium ions on a negatively charged cell membrane. Factor Xa, alone, can activate prothrombin to thrombin however, the rate of conversion is not physiologically relevant for survival. Incorporation of factor Va into prothrombinase accelerates the rate of prothrombinase activity by 300,000-fold, and provides the physiological pathway of thrombin generation. The long-term goal of …
Molecular Mechanism Of Incorporation Of Factor Va Into Prothrombinase, Melissa Barhoover
Molecular Mechanism Of Incorporation Of Factor Va Into Prothrombinase, Melissa Barhoover
ETD Archive
The mainstay of the blood coagulation cascade is the formation of the fibrin clot, catalyzed by the serine protease, thrombin. The prothrombinase complex catalyzes the activation of prothrombin to thrombin and, is composed of the enzyme, factor Xa, and the protein cofactor, factor Va, in the presence of divalent metal ions associated on a membrane surface. The enzyme, factor Xa, alone can activate prothrombin by two sequential proteolytic cleavages at Arg271 and Arg320 resulting in the intermediates, Fragment 1.2 and Prethrombin 2. The overall rate of this reaction is not compatible with survival. On the other hand, the incorporation of …