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Modified Electrodes With Grafted Dna And Oligonucleotides For Detection And Quantification Of Peroxynitrate, Heba Azmy Salim
Modified Electrodes With Grafted Dna And Oligonucleotides For Detection And Quantification Of Peroxynitrate, Heba Azmy Salim
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Peroxynitrite (ONOO-) is a strong oxidizing and nitrating agent, and its formation has been correlated with many pathological conditions. It is generated in-vivo through the diffusion-controlled reaction between nitric oxide and superoxide. Peroxynitrite has been linked to nitration of protein and DNA as well as to DNA strand breaks. Accumulation of mutations and/or other kinds of DNA damage represent a carcinogenic risk. The accurate measurement of peroxynitrite concentration has been a challenge since this analyte is very unstable and reacts with many cellular targets. Development of analytical techniques capable of rapid and sensitive detection of this fast-reacting and damaging agent …