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Full-Text Articles in Physical Sciences and Mathematics
13c-Methyl Isocyanide As An Nmr Probe For Cytochrome P450 Active Site, Christopher R Mccullough, Phani Kumar Pullela, Sang-Choul Im, Lucy Waskell, Daniel S. Sem
13c-Methyl Isocyanide As An Nmr Probe For Cytochrome P450 Active Site, Christopher R Mccullough, Phani Kumar Pullela, Sang-Choul Im, Lucy Waskell, Daniel S. Sem
Chemistry Faculty Research and Publications
The cytochromes P450 (CYPs) play a central role in many biologically important oxidation reactions, including the metabolism of drugs and other xenobiotic compounds. Because they are often assayed as both drug targets and anti-targets, any tools that provide: (a) confirmation of active site binding and (b) structural data, would be of great utility, especially if data could be obtained in reasonably high throughput. To this end, we have developed an analog of the promiscuous heme ligand, cyanide,with a 13CH3-reporter attached. This 13C-methyl isocyanide ligand binds to bacterial (P450cam) and membrane-bound mammalian (CYP2B4) CYPs. It can be …