Open Access. Powered by Scholars. Published by Universities.®
Physical Sciences and Mathematics Commons™
Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Physical Sciences and Mathematics
Functionalized Carbon Nanotube Adsorption Interfaces For Electron Transfer Studies Of Galactose Oxidase, Mulugeta B. Wayu, Michael J. Pannell, Najwa Labban, William S. Case, Julie A. Pollack, Michael C. Leopold
Functionalized Carbon Nanotube Adsorption Interfaces For Electron Transfer Studies Of Galactose Oxidase, Mulugeta B. Wayu, Michael J. Pannell, Najwa Labban, William S. Case, Julie A. Pollack, Michael C. Leopold
Chemistry Faculty Publications
Modified electrodes featuring specific adsorption platforms able to access the electrochemistry of the copper containing enzyme galactose oxidase (GaOx) were explored, including interfaces featuring nanomaterials such as nanoparticles and carbon nanotubes (CNTs). Electrodes modified with various self-assembled monolayers (SAMs) including those with attached nanoparticles or amide-coupled functionalized CNTs were examined for their ability to effectively immobilize GaOx and study the redox activity related to its copper core. While stable GaOx electrochemistry has been notoriously difficult to achieve at modified electrodes, strategically designed functionalized CNT-based interfaces, cysteamine SAM-modified electrode subsequently amide-coupled to carboxylic acid functionalized single wall CNTs, were significantly more …
First Generation Amperometric Biosensing Of Galactose With Xerogel-Carbon Nanotube Layer-By-Layer Assemblies, Najwa Labban, Mulugeta B. Wayu, Ciara M. Steele, Tess S. Munoz, Julie A. Pollock, William S. Case, Michael C. Leopold
First Generation Amperometric Biosensing Of Galactose With Xerogel-Carbon Nanotube Layer-By-Layer Assemblies, Najwa Labban, Mulugeta B. Wayu, Ciara M. Steele, Tess S. Munoz, Julie A. Pollock, William S. Case, Michael C. Leopold
Chemistry Faculty Publications
A first-generation amperometric galactose biosensor has been systematically developed utilizing layer-by-layer (LbL) construction of xerogels, polymers, and carbon nanotubes toward a greater fundamental understanding of sensor design with these materials and the potential development of a more efficient galactosemia diagnostic tool for clinical application. The effect of several parameters (xerogel silane precursor, buffer pH, enzyme concentration, drying time and the inclusion of a polyurethane (PU) outer layer) on galactose sensitivity were investigated with the critical nature of xerogel selection being demonstrated. Xerogels formed from silanes with medium, aliphatic side chains were shown to exhibit significant enhancements in sensitivity with the …