Physical Sciences and Mathematics Commons^™

Analytical Method Development For The Detection And Analysis Of Protein Carbonyls, Chelsea M. Coffey

Theses and Dissertations

Oxidative stress can result in changes to many biomolecules and also affect their activities. We are interested in protein carbonylation, a type of unnatural oxidation which has been associated with numerous degenerative disease states and is also a consequence of the natural aging process. Protein carbonyls are stable species, but countless analytical barriers exist in terms of their identification. Thus, the main goal of this work was to develop and optimize analytical methods that could be used to help us better understand which, where, and how proteins are being carbonylated.

Initial studies involved method validation for carbonylating, tagging, and enriching …