Physical Sciences and Mathematics Commons^™

Electrochemical Determination Of The Heterogeneous Electron Transfer Kinetics Of Soluble Spinach Ferredoxin, Charlene D. Crawley

Theses and Dissertations

The application of electrochemical techniques to biological systems has become an attractive method for characterizing the redox and electron transfer behavior of biomacromolecules. This study focuses on the role of soluble spinach ferredoxin (Fd) in photosynthesis where it functions as an electron carrier to membrane bound species in the plant chloroplast. An electrochemical model is used where in Fd now serves as a soluble redox species which undergoes oxidation and reduction via electron transfer at an electrode. This type of model is significant in that both the membrane and electrode reactive sites are characterized by charged bilayer interfacial regions which …

Interfacial Electrochemistry Of Cytochrome C And Myoglobin, Edmond F. Bowden

Theses and Dissertations

The interfacial electrochemistry of horse heart cytochrome c was studied at metal and metal oxide electrodes. Sperm whale myoglobin reactions at metal oxide and surface modified electrodes were also investigated. Emphasis was on the measurement and mechanistic interpretation of the heterogeneous electron transfer kinetic parameters for these electrode reactions.

The motivation for this study stems from the fact that some electron transfer proteins, e.g., cytochrome c, transfer electrons heterogeneously in physiological systems. Specific electrodes may, therefore, serve as suitable models for physiological surfaces. Electrode and membrane interfaces are both electrically charged and both have oriented hydration water layers.

The cyclic …