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Full-Text Articles in Physical Sciences and Mathematics
Biochemical Investigations Of Proteins Associated With Iron Homeostasis In Escherichia Coli, Matthew Ryan Blahut
Biochemical Investigations Of Proteins Associated With Iron Homeostasis In Escherichia Coli, Matthew Ryan Blahut
Theses and Dissertations
The regulation of transition metals within living organisms is essential to the persistence of life. Of particular interest to our lab is iron due to the prevalence of this metal within the cell in addition to the numerous functions it performs. Fe-S cofactors are a significant prosthetic group consisting of iron that are necessary for the function of various metalloproteins. Generation of Fe-S clusters depends on several biosynthesis pathways varying between organisms with Isc and Suf both utilized in E. coli. While Isc controls Fe- S manufacturing under normal conditions, Suf assumes control when the cell is exposed to …
Nickel Exposure Reduces Enterobactin Production In Escherichia Coli, Clorissa L. Washington-Hughes, Geoffrey T. Ford, Alsten D. Jones, Kimberly Mcrae, Franklin Wayne Outten
Nickel Exposure Reduces Enterobactin Production In Escherichia Coli, Clorissa L. Washington-Hughes, Geoffrey T. Ford, Alsten D. Jones, Kimberly Mcrae, Franklin Wayne Outten
Faculty Publications
Escherichia coli is a well- studied bacterium that can be found in many niches, such as industrial wastewater, where the concentration of nickel can rise to low- millimolar levels. Recent studies show that nickel exposure can repress pyochelin or induce pyo-verdine siderophore production in Pseudomonas aueroginosa. Understanding the mo-lecular cross- talk between siderophore production, metal homeostasis, and metal toxicity in microorganisms is critical for designing bioremediation strategies for metal- contaminated sites. Here, we show that high- nickel exposure prolongs lag phase duration as a result of low- intracellular iron levels in E. coli. Although E. coli cells respond to low- …
Nickel Exposure Reduces Enterobactin Production In Escherichia Coli, Clorissa L. Washington – Hughes, Geoffrey T. Ford, Alsten D. Jones, Kimberly Mcrae, Franklin W. Outten
Nickel Exposure Reduces Enterobactin Production In Escherichia Coli, Clorissa L. Washington – Hughes, Geoffrey T. Ford, Alsten D. Jones, Kimberly Mcrae, Franklin W. Outten
Faculty Publications
Escherichia coli is a well- studied bacterium that can be found in many niches, such as industrial wastewater, where the concentration of nickel can rise to low- millimolar levels. Recent studies show that nickel exposure can repress pyochelin or induce pyo-verdine siderophore production in Pseudomonas aueroginosa. Understanding the mo-lecular cross- talk between siderophore production, metal homeostasis, and metal toxicity in microorganisms is critical for designing bioremediation strategies for metal- contaminated sites. Here, we show that high- nickel exposure prolongs lag phase duration as a result of low- intracellular iron levels in E. coli. Although E. coli cells respond to low- …
Illuminating The Interactions And Functions Of Glutaredoxins, Bola Proteins, And Erv1 In Iron Homeostasis, Adrian Colleen Dlouhy
Illuminating The Interactions And Functions Of Glutaredoxins, Bola Proteins, And Erv1 In Iron Homeostasis, Adrian Colleen Dlouhy
Theses and Dissertations
Iron is a redox-active protein cofactor required for essential cellular functions such as respiration, however excess intracellular iron can generate damaging reactive oxygen species. Understanding how cells regulate iron levels is critical for treatment of human diseases that span from anemia to iron overload disorders. Glutaredoxins (Grxs) with a CGFS active site are highly conserved proteins shown to have roles in iron homeostasis and iron-sulfur cluster assembly, thus earning them the title “the Iron Whores”. They can exist either as a [2Fe-2S] cluster-bound dimer or an apo monomer, suggesting conservation of structure and function. In addition, Grxs interact with the …