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Extra-Mitochondrial Cu/Zn Superoxide Dismutase (Sod1) Is Dispensable For Protection Against Oxidative Stress But Mediates Peroxide Signaling In Saccharomyces Cerevisiae, Claudia Montllor-Albalate, Alyson E. Colin, Bindu Chandrasekharan, Naimah Bolaji, Joshua L. Andersen, Franklin W. Outten, Amit R. Reddi

Faculty Publications

Cu/Zn Superoxide Dismutase (Sod1) is a highly conserved and abundant metalloenzyme that catalyzes thedisproportionation of superoxide radicals into hydrogen peroxide and molecular oxygen. As a consequence, Sod1serves dual roles in oxidative stress protection and redox signaling by both scavenging cytotoxic superoxideradicals and producing hydrogen peroxide that can be used to oxidize and regulate the activity of downstreamtargets. However, the relative contributions of Sod1 to protection against oxidative stress and redox signaling arepoorly understood. Using the model unicellular eukaryote, Baker's yeast, we found that only a small fraction ofthe total Sod1 pool is required for protection against superoxide toxicity and that …