Physical Sciences and Mathematics Commons^™

Construction Of Synthetic Signal Pathways In Mammalian Cells Via Induced Protein Proximity, Guihua Zeng

Chemistry and Chemical Biology ETDs

Cell signaling translates extracellular signals into intracellular processes that carry out cellular functions. The introduction of artificial signaling networks using synthetic biology methods has facilitated the investigation of signaling mechanisms and the generation of novel cell functions. Several synthetic biology methods that are based on chemical controls have been developed to provide precise temporal regulation. In my dissertation, we constructed new synthetic biology engineering strategies to control tailored cellular events.

Described in Chapter 2, we developed a new chemical strategy to generate de novo signaling pathways that link a signaling molecule, H₂O₂, to different downstream cellular …