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Glycoform Analysis Of Alpha1-Acid Glycoprotein By Capillary Electrophoresis Using Electrophoretic Injection, Chenhua Zhang, William Clarke, David S. Hage
Glycoform Analysis Of Alpha1-Acid Glycoprotein By Capillary Electrophoresis Using Electrophoretic Injection, Chenhua Zhang, William Clarke, David S. Hage
David Hage Publications
Human alpha1-acid glycoprotein (AGP) is an acute phase glycoprotein that has a heterogeneous glycosylation pattern. This pattern can change in certain diseases, which has resulted in interest in using AGP glycoforms as potential biomarkers for these diseases. This report describes a method that uses capillary electrophoresis to characterize and analyze AGP glycoforms both in purified samples of AGP and in human serum. This method uses static and dynamic coatings of poly (ethylene oxide) that are applied to a silica capillary for separation of AGP glycoforms in the reversed-polarity mode of CE and in the presence of negligible electroosmotic …