Physical Sciences and Mathematics Commons^™

Developing Novel Electrospray Ionization Mass Spectrometry (Esi Ms) Techniques To Study Higher Order Structure And Interaction Of Biopolymers, Agya K. Frimpong

Open Access Dissertations

Mass spectrometry has enjoyed enormous popularity over the years for studying biological systems. The theme of this dissertation was to develop and use mass spectrometry based tools to solve five biologically oriented problems associated with protein architecture and extend the utility of these tools to study protein polymer conjugation. The first problem involved elucidating the false negatives of how proteins with few basic residues, forms highly charged ions in electrospray ionization mass spectrometry (ESI MS). This study showed that the unfolding of polypeptide chains in solution leads to the emergence of highly charged protein ions in ESI MS mass spectra, …