Physical Sciences and Mathematics Commons^™

Dinitroxides In Rapid Scan Electron Paramagnetic Resonance Imaging, Lukas B. Woodcock

Electronic Theses and Dissertations

Local tissue physiology is an important parameter in understanding disease behavior. Rapid Scan (RS) electron paramagnetic resonance (EPR) offers a unique, non-invasive tool for investigation of these so-called microenvironments through EPR Imaging (EPRI). Research into advancement of EPRI falls into many categories. Not least among those are advances in instrumentation and methodology. Presented here are updates to a benchtop EPRI instrument operating at 1 GHz targeted at pre-clinical EPRI applications. Newly developed methods for reducing RS-EPR background through inversion of the magnetic field are also demonstrated. EPR applications are limited in native biological systems due to the miniscule concentration of …

Electron Paramagnetic Resonance Imaging And Probes, Hanan B. Elajaili

Electronic Theses and Dissertations

EPR imaging at low frequency is an important method to measure in vivo physiology. Because native paramagnetic species exist at low concentrations in living systems, exogenous paramagnetic species are needed for in vivo EPR. Selection of the appropriate EPR probe allows the experimenter to obtain information about the environment of the imaged probe and its spatial distribution. Relaxation times for several nitroxide radicals were studied with the aim of understanding the relaxation mechanisms, which provides criteria for design of suitable in vivo imaging probes.

Amino-substituted trityl radicals have the potential to monitor pH in vivo, and the suitability for this …