Open Access. Powered by Scholars. Published by Universities.®
Physical Sciences and Mathematics Commons™
Open Access. Powered by Scholars. Published by Universities.®
- Keyword
Articles 1 - 12 of 12
Full-Text Articles in Physical Sciences and Mathematics
Mechanochemical Synthesis Of Fuels From Sustainable Sources Utilizing Solid Catalysts, Jacob Todd
Mechanochemical Synthesis Of Fuels From Sustainable Sources Utilizing Solid Catalysts, Jacob Todd
Electronic Theses and Dissertations
Mechanochemistry requires less solvents which results in less waste generation. The question becomes can we apply mechanochemistry to current chemical reactions and reduce the generation of waste. This process can be simple or can be very extensive resulting in the generation of large amounts of waste and very little product. A significant advantage of mechanochemistry is the reduced need for solvents that would be common place for reactions. Mechanochemistry transfers energy to reagents via impacts from milling media. Energy can be transferred to the reagents no matter their state-of-matter. Much of the current studies in mechanochemistry today are done using …
Time-Resolved Phosphorescence Spectroscopy At Cryogenic Temperatures For The Environmental Analysis Of Polycyclic Aromatic Sulfur Heterocycles In Oil Contaminated Sites., Maha Al-Tameemi
Electronic Theses and Dissertations
The large volume of crude oil released into the Gulf of Mexico by the Deepwater Horizon (DWH) accident has raised considerable concerns over potential ecosystem impacts. The dispersion of harmful oil components into the ocean waters could pose long term risks to flora and fauna. Due to the complexity of oil contaminated sites, the unambiguous identification and quantitation of environmental pollutants often requires the sequence of high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS). A classic example is the analysis of the sixteen polycyclic aromatic hydrocarbons included in the priority pollutants list of the U.S. Environmental Protection Agency (EPA-PAHs). …
A Universal Electrochemical Biosensor For The Detection Of Nucleic Acids Based On A Four-Way Junction Structure, Dawn Mills
Electronic Theses and Dissertations
Electrochemical hybridization sensors have been explored extensively for analysis of specific nucleic acids. However, commercialization of the platform is hindered by the need for attachment of separate oligonucleotide probes complementary to a RNA or DNA target to an electrode's surface. This dissertation demonstrates that a single probe can be used to analyze several nucleic acid targets of different lengths with high selectivity, low cost and can be regenerated in 30 seconds by a simple water rinse. The universal electrochemical four-way junction (4J)-forming (UE4J) sensor consists of a universal DNA stem-loop (USL) probe attached to the electrode's surface and two adaptor …
Two-Component Covalent Inhibitors (Tcci) Of The Human Immunodeficiency Virus Reverse Transcriptase (Hiv-Rt), Carlos Ledezma
Two-Component Covalent Inhibitors (Tcci) Of The Human Immunodeficiency Virus Reverse Transcriptase (Hiv-Rt), Carlos Ledezma
Electronic Theses and Dissertations
The traditional design of nucleoside reverse transcriptase inhibitors (NRTI's) involves the synthesis of chain-terminated nucleoside analogs. HIV-RT has relatively low fidelity which facilitates mutations that confer resistance towards NRTI's, also, drug promiscuity from NRTI's result in various side-effects that lead to poor patient adherence to treatment. We designed and tested two-component covalent inhibitors against HIV-RT. Our inhibitor design results in higher specificity due to its binary approach, which has previously been used in biosensing applications, where both components are necessary for therapeutic effect, and lower chances for mutagenesis because of its inhibitory action. The TCCI approach results in up to …
Monitoring Crystal Structure Refinements Using Solid-State Nmr Chemical Shift Tensors, Keyton Kalakewich
Monitoring Crystal Structure Refinements Using Solid-State Nmr Chemical Shift Tensors, Keyton Kalakewich
Electronic Theses and Dissertations
Inclusion of lattice-fields in density functional theory (DFT) methods has enabled the accurate calculation of solid-state nuclear magnetic resonance (SSNMR) chemical shift tensors. Calculated 13C and 15N tensors (i.e. 3 principle values per nucleus) can be used to monitor crystal structure refinements and to select the correct structure from a large population of computationally generated candidates. In this dissertation, chapter 2 describes a methodology to improve established crystal structures from three different diffraction techniques involving geometric refinement monitored using SSNMR tensor values. The calculated 13C tensors for three relatively simple organic compounds (i.e. acetaminophen, naphthalene, and adenosine) are shown to …
Nitrogen-Containing Materials For Mechanochemical Synthesis, Luminescence Analysis, And Heterogeneous Catalysis, David Nash
Electronic Theses and Dissertations
Various inorganic nitrogen-containing materials have been exploited for their different properties. Several nitride materials are commercially attractive due to their mechanical properties making them suitable for ceramic industries and wide bandgaps fitting for use as semiconductor and insulator materials, as well as optoelectronics. Nitride materials can exhibit versatility in applications such as the use of gallium nitride to make blue LEDs, nitrides of titanium and silicon being utilized as medical implants for their chemical inertness and hardness, and the heavy use of boron nitride as a solid lubricant in the cosmetic industry. Amines have been used as nitrogen-containing organic ligands …
Detection Of Inorganic Phosphate In Environmental Water Samples Using A Terbium And Gold Nanoparticle-Based Fret Chemosensor, Madeleine Johnson
Detection Of Inorganic Phosphate In Environmental Water Samples Using A Terbium And Gold Nanoparticle-Based Fret Chemosensor, Madeleine Johnson
Electronic Theses and Dissertations
A novel chemosensor for the detection of inorganic phosphate (Pi) in environmental water samples is outlined. The sensing solution is comprised of a luminescent lanthanide, terbium (Tb3+), chelated to ethylenediaminetetraacetic acid (EDTA) acid in solution with cetyltrimethylammonium bromide (CTAB)- capped gold nanoparticles (AuNPs). The Tb-EDTA and AuNPs undergo a Fluorescence resonance energy transfer (FRET) mechanism in which the Tb3+ luminescence is quenched. Upon the addition of inorganic phosphate (Pi), the AuNPs begin to aggregate and precipitate out of solution. The aggregation of AuNPs results in the restoration of the Tb-EDTA signal which can then be correlated to Pi concentration in …
A Deoxyribozyme Sensor And Isothermal Amplification For Human Sex Determination, Alexandra Smith
A Deoxyribozyme Sensor And Isothermal Amplification For Human Sex Determination, Alexandra Smith
Electronic Theses and Dissertations
Ribozymes are known to catalyze biochemical reactions and behave like enzymes. They are naturally occurring and have very diverse functions within a cell. After investigating ribozymes that next step was to find if DNA can exhibit the same characteristics since RNA and DNA only differ by a ribose 2'-hydroxyl group. This evolution in curiosity gave rise to artificial DNA enzymes that can catalyze certain reactions and have been created by in vitro selection methods. Due to the ability to manipulate and control DNA hybridization, the deoxyribozyme is advantageous to the field of molecular diagnostics. Other hybridization probes like Taqman for …
Using Low-Coherence Interferometry To Monitor Cell Invasion In An In-Vitro Model System, Behnaz Davoudi Nasab
Using Low-Coherence Interferometry To Monitor Cell Invasion In An In-Vitro Model System, Behnaz Davoudi Nasab
Honors Undergraduate Theses
In an optically random system, such as naturally occurring and man-made media, light undergoes pronounced multiple scattering. This phenomenon has shown a remarkable potential in characterizing complex materials. In this regime, scattering occurs from each individual center of the scattering and independent scattering events lead to multiple light scattering. This phenomenon is often described as a random walk of photons and can be modeled in terms of a diffusion equation based on the radiative transfer theory. In this thesis, we used optical path-length spectroscopy (OPS), which is an experimental method to obtain the path-length probability density of the propagating light …
Micro-Spectroscopy Of Bio-Assemblies At The Single Cell Level, Jeslin Kera
Micro-Spectroscopy Of Bio-Assemblies At The Single Cell Level, Jeslin Kera
Honors Undergraduate Theses
In this thesis, we investigate biological molecules on a micron scale in the ultraviolet spectral region through the non-destructive confocal absorption microscopy. The setup involves a combination of confocal microscope with a UV light excitation beam to measure the optical absorption spectra with spatial resolution of 1.4 μm in the lateral and 3.6 μm in the axial direction. Confocal absorption microscopy has the benefits of requiring no labels and only low light intensity for excitation while providing a strong signal from the contrast generated by the attenuation of propagating light due to absorption. This enables spatially resolved measurements of single …
Measuring And Modeling Nmr And Emission Spectra To Gain New Insight Into Challenging Organic Compounds, Jacob Powell
Measuring And Modeling Nmr And Emission Spectra To Gain New Insight Into Challenging Organic Compounds, Jacob Powell
Electronic Theses and Dissertations
The advancement of theoretical methods in recent years has allowed the calculation of highly accurate spectroscopic parameters. Comparing these values to the corresponding experimental data can allow molecular structures to be elucidated. This dissertation details the use of experimental and theoretical data from nuclear magnetic resonance (NMR) and fluorescence spectroscopy to determine structure. Herein the NMR focus is on measuring & modeling chemical shift anisotropy and one-bond carbon-carbon J-coupling constants (1JCC). The fluorescence analysis models vibrationally resolved fluorescence spectra. Chemical shift anisotropy techniques were used to study two conflicting crystal structures of the n-alkyl fatty acid, lauric acid. These two …
Literature Review On The Use Of Nucleic Acid-Based Logic Gates For The Detection Of Human Diseases, Enrique J. Blanco Martinez
Literature Review On The Use Of Nucleic Acid-Based Logic Gates For The Detection Of Human Diseases, Enrique J. Blanco Martinez
Honors Undergraduate Theses
Conventional methods for diagnosis of human disease are, at times, limited in different regards including time requirement, either experimental or data processing, sensitivity, and selectivity. It is then that a Point of Care Criteria, which considers the true utility and usefulness of the device, is employed to propose new diagnostic devices capable of overcoming the aforementioned shortcomings of conventional tools. Nucleic acid, characterized for its predictable base-pairing nature, is considered to be a highly-selective, yet greatly modifiable device. Its behavior is then described through Boolean Logic, where “true” or “false” outputs are mathematically described as “1” and “0”, respectively. This …