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Full-Text Articles in Physical Sciences and Mathematics
Stability Of E. Coli Atcase And Its Catalytic Subunit Measured By Intrinsic Protein Fluorescence, Ira A. Gurland
Stability Of E. Coli Atcase And Its Catalytic Subunit Measured By Intrinsic Protein Fluorescence, Ira A. Gurland
Honors Theses
Intrinsic protein fluorescence has been used to monitor the guanidine HCI induced denaturation of E. coli aspartate transcarbamylase (ATCase) and its isolated catalytic subunit (CSU). ATCase is a large oligomeric protein which contains both regulatory subunits and catalytic subunits. Both tryptophans in ATCase are located in a single globular domain of the catalytic subunit. For both ATCase and CSU, increasing concentralions of guanidine HCI caused a red shift of the maximum protein emission (Aex 295 nm) of 15.0 +/- 0.5 nm. CSU showed a concommitant decrease in fluorescence intensity while ATCase showed an increase from 0.0 to 2.0 M and …
The Construction Of An Apparatus To Do Fast Transient Ir Spectroscopy, Eugene Jerome Bernard
The Construction Of An Apparatus To Do Fast Transient Ir Spectroscopy, Eugene Jerome Bernard
Honors Theses
Photocatalytic reactions of metal carbonyl complexes with organic substrates have been studied extensively over the past decade. The reaction mechanisms proposed for most reactions studied have come from varying the concentrations and conditions on the reactants and examining the effects on the prc,ducts. The intermediate steps of the reaction mechanism are inferred from these kinds of experiments, but most intermediates are too short lived to be seen directly with conventional IR spectroscopy. This thesis describes the construction of an apparatus that uses fast transient IR spectroscopy to study the reaction intermediates that are too short lived to be detected by …