Physical Sciences and Mathematics Commons^™

Analysis Of Nanoparticle Transmission Electron Microscopy Data Using A Public- Domain Image-Processing Program, Image, Gerd H. Woehrle, James E. Hutchison, Sai̇m Özkar, Richad G. Finke

Turkish Journal of Chemistry

The need to easily and quickly count larger numbers of nanoparticles, in order to obtain statistically useful size and size-distribution data, is addressed via the use of a readily available, free, public-domain program for particle counting, NIH-Image (and 2 others derived from it, Scion Image and Image J), collectively referred to herein as Image. The best protocols that we have found useful for the use of Image are reported; both appropriate as well as problematic applications of Image are then illustrated with a series of TEM images of Ir(0), Pd(0) and Au(0) nanoclusters. Methods to detect and image nanoclusters with …