Open Access. Powered by Scholars. Published by Universities.®
Physical Sciences and Mathematics Commons™
Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 4 of 4
Full-Text Articles in Physical Sciences and Mathematics
Evaluating The Effect Of Resveratrol On Sirt1 With Different Peptide Substrates, Yujin Hur
Evaluating The Effect Of Resveratrol On Sirt1 With Different Peptide Substrates, Yujin Hur
Master's Theses
SIRT1 is an NAD+-dependent lysine deacetylase that is involved in many important cellular pathways. The protein has an extended N-terminal domain that seems to play a role in regulating its activity. Sirtuin Activating Compounds (STACs) are allosteric activators, such as resveratrol, that regulate the activity of SIRT1. STACs bind to the N-terminal STAC Binding Domain (SBD) in SIRT1. Past literature has found that SIRT1 activity can be increased, decreased, or remain unchanged by resveratrol depending on the identity of the peptide substrate. The goal of this study is to clarify the mechanism for substrate-specific allosteric regulation by resveratrol. The interactions …
Characterization And Evaluation Of Catalytic Activity Of Small Molecule Copper Complexes For Metallopolymer Design, Brenda Lu
Master's Theses
Catalysis is one of the driving forces in designing greener chemical processes for essential real-world applications. Enzymes, Nature’s catalysts, are known for their high selectivity, use of readily available metals and reagents, and ability to work under physiological conditions; three important qualities that synthetic chemists would like to mimic in their small molecule metal catalysts. Though most small molecule metal catalysts fall short of enzyme-like activity, we hypothesize that using a polymer scaffold as a secondary coordination sphere mimic around the metal complex will affect the microenvironment (steric and electronic) and enable enzyme-like activity. Previously, we synthesized ligand precursors and …
Optimizing Soluble Recombinant Expression Of The Aedes Aegypti Early Trypsin Protease, Neomi Millan
Optimizing Soluble Recombinant Expression Of The Aedes Aegypti Early Trypsin Protease, Neomi Millan
Master's Theses
The female Aedes aegypti mosquito is a primary vector for the transmission of blood–borne viral pathogens such as Chikungunya, Zika, Yellow Fever, and Dengue. The female mosquito will become infected with these pathogens through the uptake of a blood meal from an infected host. The midgut digestive enzymes are required to digest the blood meal to obtain necessary nutrients needed for the completion of the gonotrophic cycle. Blood meal digestion is biphasic and digestive enzymes are expressed at different time points after uptake of a blood meal. The focus of this study is on an early phase protease known as …
Single Chain Nanoparticles As Synthetic Enzyme Mimics, Tony Mo
Single Chain Nanoparticles As Synthetic Enzyme Mimics, Tony Mo
Master's Theses
Enzymes, nature’s catalysts, are a crucial component in biological reactions because they help lower the activation energy and speed up these reactions. They display impressive selectivity, catalytic activity, and specificity. However, they can be difficult to produce synthetically, especially on a large scale, and they are not suitable for all reactions for which synthetic catalysts are needed. Metalloenzymes contain a transition metal active site inside the primary coordination sphere (PCS) (ligands directly bound to the metal) that is surrounded by a secondary coordination sphere (SCS) (made up of more distant amino acids) which provides steric bulk and intramolecular interactions between …