Open Access. Powered by Scholars. Published by Universities.®
Physical Sciences and Mathematics Commons™
Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Physical Sciences and Mathematics
Fluorescence Probes For Cellular Thiol And Disulfide Detection: Sythesis And Biophysical Characterization, Timothy Jonhera
Fluorescence Probes For Cellular Thiol And Disulfide Detection: Sythesis And Biophysical Characterization, Timothy Jonhera
Dissertations (1934 -)
The total concentration of cellular thiols is about 30 mM, and use of current fluorescent chemical probes to quantitate these thiols inside cells is impractical. We recently reported fluorescent dithio probes (donor-S-S-acceptor, DSSA) with unusually low reduction potential (- 0.6 V) that can detect changes in intracellular thiols and cross membranes of bacterial and mammalian cells, but they could not quantify thiol redox state. Here we report a new variant of the DSSA series, which has hydroxy coumarin (Ex: 320 nm, Em: 460 nm) as donor and fluorescein (Ex: 485 nm, Em: 520 nm) as acceptor. The probe was characterized …