Open Access. Powered by Scholars. Published by Universities.®
Physical Sciences and Mathematics Commons™
Open Access. Powered by Scholars. Published by Universities.®
- Publication
Articles 1 - 3 of 3
Full-Text Articles in Physical Sciences and Mathematics
Purification And Analysis Of Protein Synthetic Initiation Factors From Volvox Carteri, Kathie L. Pooler
Purification And Analysis Of Protein Synthetic Initiation Factors From Volvox Carteri, Kathie L. Pooler
Honors Theses
Volvox carteri, a multi-celled green algae, can grow synchronously given a sixteen hour light period followed by an eight hour dark period, a cycle which is repeated for a 48 hour growth cycle total. Near the end of each light period, reproductive cells divide rapidly resulting in the differentiation of ceIls. When the dark period begins, this differentiation stops and the cells remain dormant with little protein synthesis or differentiation occurring. Immediately after the lights come back on, however, the cells again undergo rapid protein synthesis and complete their differentiation. Previous studies have concluded that Volvox carteri discontinue protein synthesis …
Isolation Of Protein Synthesis Initiation Factors Using Volvox Carteri: A Clue To The Translational Control Of Cytodifferentiation, Claudia Hackethal
Isolation Of Protein Synthesis Initiation Factors Using Volvox Carteri: A Clue To The Translational Control Of Cytodifferentiation, Claudia Hackethal
Honors Theses
Volvox carteri, a multicellular green algae, possesses two distinct cell types--the gonidia and somatic cells. This simple germ-soma dichotomy is ideal for the study of the control of cytodifferentiation. The control of cytodifferentiation in this organism has been identified as a posttranscriptional mechanism, which suggests translation. Initiation factors have been shown to play an important role in controlling translation in many other developmental systems. Therefore, study of the initiation factors in Volvox may shed light on a translational control mechanism. In order to study any modifications of these factors, they must be isolated, possibly by following an adapted wheat germ …
Two-Dimensional Gel Electrophoretic Analysis Of Cellular Proteins From Escherichia Coli In The Presence Of Mutated And Homologous Genes For 4.5s Rna, Michael O'Loughlin
Two-Dimensional Gel Electrophoretic Analysis Of Cellular Proteins From Escherichia Coli In The Presence Of Mutated And Homologous Genes For 4.5s Rna, Michael O'Loughlin
Senior Scholar Papers
4.5S RNA is a small. stable, required molecule in Escherichia coli whose precise function is not known. Previous evidence suggests an involvement with the ribosome during translation. In this study, four base insertion and four base deletion mutations were constructed in a plasmid borne copy of the 4.55 RN A gene. These copies and two homologous genes from Pseudomonas aeruginosa and Thermus thermophilus were transformed into an E. coli strain which then had its only wild-type 4.5S RNA gene inactivated. In order to assess the effect of the alternative gene copies. the patterns of proteins synthesized in the presence of …