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Full-Text Articles in Physical Sciences and Mathematics
Characterization Of The 4.5s Rna Molecule In Escherichia Coli, Bruce John Panilaitis
Characterization Of The 4.5s Rna Molecule In Escherichia Coli, Bruce John Panilaitis
Senior Scholar Papers
The 4.5S RNA molecule of Escherichia coli is essential to cell viability. It has been shown that depletion of this molecule inhibits protein synthesis, induces the heat shock response, and generally slows cell growth. The molecule has also been implicated in protein secretion, as in cells depleted of 4.5S RNA, an unsecreted precursor to ?-lactamase accumulates (pre-?-lactamase). A role in protein secretion is further supported by structural similarities with the 7S RNA molecule of eukaryotic SRP, specific binding to SRP54, and its homolog in E. coli, P48, and the ability of 7S RNA from certain archaebacteria to suppress 4.5S RNA …
Purification And Analysis Of Protein Synthetic Initiation Factors From Volvox Carteri, Kathie L. Pooler
Purification And Analysis Of Protein Synthetic Initiation Factors From Volvox Carteri, Kathie L. Pooler
Honors Theses
Volvox carteri, a multi-celled green algae, can grow synchronously given a sixteen hour light period followed by an eight hour dark period, a cycle which is repeated for a 48 hour growth cycle total. Near the end of each light period, reproductive cells divide rapidly resulting in the differentiation of ceIls. When the dark period begins, this differentiation stops and the cells remain dormant with little protein synthesis or differentiation occurring. Immediately after the lights come back on, however, the cells again undergo rapid protein synthesis and complete their differentiation. Previous studies have concluded that Volvox carteri discontinue protein synthesis …
Investigations Of Iron Cycling In Aquatic Systems, Heather A. Lounsbury
Investigations Of Iron Cycling In Aquatic Systems, Heather A. Lounsbury
Honors Theses
A fully automated luminal-based chemiluminescence flow injection analysis system has been developed for rapid detection of Fe(IT) at natural levels in natural water systems. Similar detection limits are achieved for H202 by fluorometry using an enzyme mediated oxidation of the reagent p-hydroxyphenylacetic acid. Using these analytical systems, the rates of Fe(IT) oxidation by dissolved oxygen in 0.7 M NaO have been measured for nanomolar concentrations of Fe(II) over the pH range 7.0 to 8.3. When the production and decomposition of H202 in the system were considered, measured rates were in excellent agreement with a model based on previously reported rate …