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Plasma Biomarkers For Age-Related Macular Degeneration, Jiaqian Ni
Plasma Biomarkers For Age-Related Macular Degeneration, Jiaqian Ni
ETD Archive
No abstract provided.
Technologies For Proteomic And Genomic Biomarker Analysis, Yiding Liu
Technologies For Proteomic And Genomic Biomarker Analysis, Yiding Liu
ETD Archive
In the first part of this dissertation, we systematically validated the application of molecular weight cut-off ultrafiltration in separation and enrichment of low-molecular-weight peptides from human serum. Under optimized conditions, both free-phase and bound LMW peptides could be separated and enriched. The method proved to be highly efficient and reproducible coupled with MALDI-TOF MS proteomic pattern analysis. Three marker peaks were found to be eligible for distinguishing normal and ovarian cancer samples. A novel organic solvent precipitation method coupled with enzymatic deglycosylation was also developed for biomarker detection from human serum. This method allowed us to generate reproducible free-phase peptide …