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Full-Text Articles in Physical Sciences and Mathematics
Development Of Quantitative Bioanalytical Methods For The Measurement Of Pharmaceutical Compounds Via Hplc-Uv And Hplc-Ms/Ms, Melissa A. Mcculloch
Development Of Quantitative Bioanalytical Methods For The Measurement Of Pharmaceutical Compounds Via Hplc-Uv And Hplc-Ms/Ms, Melissa A. Mcculloch
ETD Archive
Research in pharmaceutical treatments for illnesses and disease processes has rapidly grown in the past decade. The field is fueled by advances in our understanding of the molecular mechanics of the human body, coupled with the development of sophisticated scientific instruments which can efficiently generate massive amounts of important data. This has led to an ever-growing need for the development of quantitative bioanalytical methods to measure pharmaceutical compounds. In this work, the theory behind the analytical processes and instruments that make these investigations possible is discussed. Further, the development of two high performance liquid chromatographic (HPLC) methods with both ultra …
Development Of A Novel Gradient Chromatofocusing Tandem Mass Spectometry Technique For The Determination Of Cationic Compounds In Biofluids;Identification Of Caspace 3 Cleavage Sites Of Nhe-1 By High Performance Liquid Chromatography- Mass Spectrometry, Jianhua Tang
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In Part I, work was done to develop a novel gradient chromatofocusing liquid chromatography-tandem mass spectrometry (MS/MS) technique for the determination of ionic compounds in biological matrix. The analysis of ionic compounds poses a great challenge to current LC-MS techniques, due to the fact that there is little or no retention. These ionic compounds are eluted at almost the same time as biological matrix, which is normally directed to waste prior to connecting to the mass spectrometer. A review of current LC-MS techniques for ionic compounds is given in Chapter 1. They all have advantages and disadvantages. To retain and …