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Isolation And Characterization Of Extracellular Vesicles From Various Biological Matrices Using Capillary-Channeled Polymer (C-Cp) Fiber Solid-Phase Extraction Spin-Down Tips, Kaylan Jackson
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A number of recent works have emphasized the need to isolate nanometer-scale analytes, like extracellular vesicles (EVs), from various biologically-relevant fluids. Exosomes are a subset of small EVs that range from 30-200 nm in diameter that serve as biomolecular snapshots of their cell of origin containing mother cell-specific DNA, miRNA, mRNA, and proteins. As critical components of intercellular communication, exosomes and other EVs play significant roles in many physiological and pathological processes. Diverse populations of these vesicles can be collected from biofluids, including blood, saliva, and urine, from cell culture conditioned media and primary cells, and even from plant …
Hydrodynamic Evaluation And Characterization Of Capillary Channeled Polymer (C-Cp) Fibers As A Stationary Phase In Hplc Of Macromolecules, Kelum Randunu Pathirannahalage
Hydrodynamic Evaluation And Characterization Of Capillary Channeled Polymer (C-Cp) Fibers As A Stationary Phase In Hplc Of Macromolecules, Kelum Randunu Pathirannahalage
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High Performance Liquid Chromatography (HPLC) is the most used analytical technique for the separation of samples in solution. HPLC of has progressed with successive use of new supports such as silica, polysaccharides, monoliths, and organic polymers. Although porous silica phases are very effective in small molecule separations, they suffer from certain drawbacks including pH stability, chemical robustness, high backpressure, and slow mass transfer for macromolecule separations. Thus, the most protein separations are carried out using nonporous, partially porous, and superficially porous phases to overcome mass transfer limitations. As a competing methodology, fiber based polymer support/stationary phases are also developed for …
Fundamental Aspects Of Small Molecules And Macromolecules On Poly(Ethylene Terephthalate) Capillary-Channeled Polymer Fibers, Christine Straut
Fundamental Aspects Of Small Molecules And Macromolecules On Poly(Ethylene Terephthalate) Capillary-Channeled Polymer Fibers, Christine Straut
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Stationary phases and adsorbents are continually being developed to enhance separations in high performance liquid chromatography (HPLC). Polymeric stationary phases have gained popularity due to their ability to be employed over a large pH range and because they are more chemically robust when compared to silica based phases. Capillary-channeled polymer (C-CP) fibers are an alternative to traditional porous packed bed beads. These fibers have a unique geometry with increased the surface area/volume ratios when compared to cylindrical fibers. Very unique characteristics are realized in the use of the C-CP fibers, including drastically reduced backpressures and selection of solute-surface interaction through …