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Development Of A Fluorescent Probe For Determination Of Water Transport In Subcellular Organelles, Adriana Chaurra-Arboleda

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Water transport has been widely studied in a variety of cells and isolated membranes. However, the intrinsic complexity related with the study of intracellular processes has delayed the development of an analytical technique for the study of water transport in subcellular compartments of living cells. This study represents the development and application of a ratiometric fluorescent probe to study water transport in endocytic organelles of intact living cells. The probe is based on the use of a D₂O-sensitive fluorophore, Lucifer yellow dextran (LY-dex), together with a D₂O-insensitive fluorophore, Alexa fluor 546 dextran (AF546-dex), used as an …