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Full-Text Articles in Physical Sciences and Mathematics
Epr Studies Of Curvature-Dependent Conformational Equilibrium In Full-Length Influenza A M2 Protein, D. Stuart Arbuckle Jr, '17
Epr Studies Of Curvature-Dependent Conformational Equilibrium In Full-Length Influenza A M2 Protein, D. Stuart Arbuckle Jr, '17
Senior Theses, Projects, and Awards
Matrix 2 (M2) is a homotetrameric integral membrane protein of the influenza A virus demonstrated to be required for generation of membrane curvature resulting in viral budding. The C-terminal amphipathic helix of M2 has been shown to generate negative Gaussian membrane curvature, required at the neck of a budding virion, dependent on membrane composition. In this thesis, electron paramagnetic resonance (EPR) spectroscopy is used to investigate the conformational dynamics of M2's amphipathic helix in liposomes consisting of DOPE and DOPC, lipids of varying intrinsic curvature propensity, as well as cholesterol enrichment. The amphipathic helix is demonstrated to populate two conformations, …
Diastereoselective Transformations And Intramolecular Cyclizations Of Enantiomerically Pure Planar Chiral N-Oxazolidinoyl Diene Lron(O) Tricarbonyl Complexes, Sooyun Choi , '17
Diastereoselective Transformations And Intramolecular Cyclizations Of Enantiomerically Pure Planar Chiral N-Oxazolidinoyl Diene Lron(O) Tricarbonyl Complexes, Sooyun Choi , '17
Senior Theses, Projects, and Awards
Organoiron complexes have established their utility in organic synthesis as stereodirecting groups as well as diene protecting groups. Since its first discovery in 1930, the diene tricarbonyl iron(O) complex underwent an impressive development that allows us to use its rich chemistry to accomplish complex molecule synthesis and to explore various synthetic methods that would broaden the scope of this field. The Paley laboratory utilizes the planar chirality of N-oxazolidinoyl diene tricarbonyl iron complexes to expand the boundaries of diastereoselective synthesis of novel compounds. The findings reported here reflect our attempts not only to reiterate the successful chemistry previously done …
Investigation Of Ai-2 Binding To The Quorum Sensing Receptor Lsrb, Meghann R. Kasal , '17
Investigation Of Ai-2 Binding To The Quorum Sensing Receptor Lsrb, Meghann R. Kasal , '17
Senior Theses, Projects, and Awards
Bacterial cell-cell communication, known as quorum sensing, is a density-dependent phenomenon that regulates multicellular behaviors such as bioluminescence and virulence. Interspecies quorum sensing is mediated by a signal known as autoinducer-2 (AI-2), which belongs to a family of molecules derived from 4,5-Dihydroxy-2,3 pentanedione (DPD), a key metabolic product of the enzyme LuxS. AI-2 is recognized by many bacteria that belong to several phyla, including Enterobacteriaceae, Rhizobiaceae, and Bacillaceae, as a non-borated cyclic derivative of DPD known as (2R,4S)-2-methyl-2,3,3,4-tetrahydroxytetrahydrofuran (R-THMF). Building on previous research, we have recently identified several novel LsrB orthologs through sequence alignments and fold-prediction software. …
Sdsl-Epr Of Residues 60-70 Of Influenza M2 Protein Reveals Structural Properties Of A Region Involved In Viral Budding, Grace Kim , '17
Sdsl-Epr Of Residues 60-70 Of Influenza M2 Protein Reveals Structural Properties Of A Region Involved In Viral Budding, Grace Kim , '17
Senior Theses, Projects, and Awards
The M2 protein is a 97-residue multifunctional transmembrane homotetrameric protein. It is found in the viral coat of the Influenza A virus, which is responsible for approximately 250,000 to 500,000 deaths annually worldwide.¹ Extensive research has elucidated M2's role as an ion channel, which plays a critical role in the uncoating of the viron once it has entered the host cell. The C-terminal tail of M2 has also been shown to play a crucial role in generating curvature in the cell membrane to mediate viral budding.²³ Deletions in the C-terminal tail of the M2 protein lead to impaired viral infectivity.⁴ …
Exploring Cyclizations Of Planar Chiral Η-4 Iron(0) Tricarbonyl Diene Complexes, Mason L. Yu , '17
Exploring Cyclizations Of Planar Chiral Η-4 Iron(0) Tricarbonyl Diene Complexes, Mason L. Yu , '17
Senior Theses, Projects, and Awards
We diastereoselectively synthesize η-4 iron(0) tricarbonyl diene complexes with the use of the oxazolidinone chiral auxiliary. The main focus of this project is to induce cyclizations via three synthetic pathways: the intramolecular Diels-Alder reaction, ring-closing enyne metathesis, and the gold(I) catalyzed methoxycyclization. Although we do not succeed in inducing these cyclizations, we demonstrate the compatibility of the iron(0) tricarbonyl complex with gold(I), and chromium(II) reagents.
Conformational Studies Of A Domain Of The Influenza A M2 Protein Involved In Viral Budding And Morphology, Alice L. Herneisen , '17
Conformational Studies Of A Domain Of The Influenza A M2 Protein Involved In Viral Budding And Morphology, Alice L. Herneisen , '17
Senior Theses, Projects, and Awards
In my two years working in the Howard lab, I have cycled through a number of projects. These projects fall under the broad context of studying of how the cytoplasmic tail of a particular membrane protein of the influenza A virus, the Matrix protein 2 (M2), is involved in viral budding. I have chosen to present each project in the form of an independent manuscript, with separate Introduction, Experimental, Results, and Discussion sections. Some broader background and theory is briefly provided in Chapters 1 and 2, but Chapters 3-5 are designed to be largely self-contained. Potential future directions pertaining to …