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Full-Text Articles in Physical Sciences and Mathematics
Identification Of Arabidopsis Gpat9 (At5g60620) As An Essential Gene Involved In Triacylglycerol Biosynthesis, Jay Shockey, Anushobha Regmi, Kimberly Cotton, Neil Adhikari, John Bowse, Philip D. Bates
Identification Of Arabidopsis Gpat9 (At5g60620) As An Essential Gene Involved In Triacylglycerol Biosynthesis, Jay Shockey, Anushobha Regmi, Kimberly Cotton, Neil Adhikari, John Bowse, Philip D. Bates
Faculty Publications
The first step in the biosynthesis of nearly all plant membrane phospholipids and storage triacylglycerols is catalyzed by a glycerol-3-phosphate acyltransferase (GPAT). The requirement for an endoplasmic reticulum (ER)-localized GPAT for both of these critical metabolic pathways was recognized more than 60 years ago. However, identification of the gene(s) encoding this GPAT activity has remained elusive. Here, we present the results of a series of in vivo, in vitro, and in silico experiments in Arabidopsis (Arabidopsis thaliana) designed to assign this essential function to AtGPAT9. This gene has been highly conserved throughout evolution and is …
Supporting Data For The Characterization Of Pna-Dna Four-Way Junctions, Douglas Van Iverson Ii, Crystal C. Serrano, Ann Marie Brahan, Arik Shams, Filbert Totsingan, Anthony J. Bell Jr.
Supporting Data For The Characterization Of Pna-Dna Four-Way Junctions, Douglas Van Iverson Ii, Crystal C. Serrano, Ann Marie Brahan, Arik Shams, Filbert Totsingan, Anthony J. Bell Jr.
Faculty Publications
Holliday or DNA four-way junctions (4WJs) are cruciform/bent structures composed of four DNA duplexes. 4WJs are key intermediates in homologous genetic recombination and double-strand break repair. To investigate 4WJs in vitro, junctions are assembled using four asymmetric DNA strands. The presence of four asymmetric strands about the junction branch point eliminates branch migration, and effectively immobilizes the resulting 4WJ. The purpose of these experiments is to show that immobile 4WJs composed of DNA and peptide nucleic acids (PNAs) can be distinguished from contaminating labile nucleic acid structures. These data compare the electrophoretic mobility of hybrid PNA–DNA junctions vs. …
An Enantiodivergent Synthesis Of CΑ-Methyl Nipecotic Acid Analogues From δ-Lactam Derivatives Obtained Through A Highly Stereoselective Cyclization Strategy, Souvik Banerjee, Emily R. Vogel, Daniel Hinton, Michael Sterling, Douglas S. Masterson
An Enantiodivergent Synthesis Of CΑ-Methyl Nipecotic Acid Analogues From δ-Lactam Derivatives Obtained Through A Highly Stereoselective Cyclization Strategy, Souvik Banerjee, Emily R. Vogel, Daniel Hinton, Michael Sterling, Douglas S. Masterson
Faculty Publications
A stereoselective and enantiodivergent strategy for the construction of δ-lactams is described. The strategy utilizes chiral malonic esters prepared from enantiomerically enriched mono esters of disubstituted malonic acid. A cyclization occurs with the selective displacement of a substituted benzyl alcohol as the leaving group. The resulting δ-lactams are then converted into nipecotic acid analogues using straightforward transformations. The resulting nipecotic acid analogues proved capable organocatalysts in Mannich reactions.
Structural Characterization Of A Newly Identified Component Of Α-Carboxysomes: The Aaa+ Domain Protein Csocbbq, Markus Sutter, Evan W. Roberts, Raul C. Gonzalez, Cassandra Bates, Salma Dawoud, Kimberly Landry, Gordon C. Cannon, Sabine Heinhorst, Cheryl A. Kerfeld
Structural Characterization Of A Newly Identified Component Of Α-Carboxysomes: The Aaa+ Domain Protein Csocbbq, Markus Sutter, Evan W. Roberts, Raul C. Gonzalez, Cassandra Bates, Salma Dawoud, Kimberly Landry, Gordon C. Cannon, Sabine Heinhorst, Cheryl A. Kerfeld
Faculty Publications
Carboxysomes are bacterial microcompartments that enhance carbon fixation by concentrating ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) and its substrate CO2 within a proteinaceous shell. They are found in all cyanobacteria, some purple photoautotrophs and many chemoautotrophic bacteria. Carboxysomes consist of a protein shell that encapsulates several hundred molecules of RuBisCO and contain carbonic anhydrase and other accessory proteins. Genes coding for carboxysome shell components and the encapsulated proteins are typically found together in an operon. The α-carboxysome operon is embedded in a cluster of additional, conserved genes that are presumably related to its function. In many chemoautotrophs, products of the expanded carboxysome …
Preparation And Preliminary Dielectric Characterization Of Structured C60-Thiol-Ene Polymer Nanocomposites Assembled Using The Thiol-Ene Click Reaction, Hanaa Mohammed Ahmed, Amber Danielle Windham, Maryam M. Al-Ejji, Noora H. Al-Qahtani, Mohammad K. Hassan, Kenneth A. Mauritz, Randy K. Buchanan, J. Paige Buchanan
Preparation And Preliminary Dielectric Characterization Of Structured C60-Thiol-Ene Polymer Nanocomposites Assembled Using The Thiol-Ene Click Reaction, Hanaa Mohammed Ahmed, Amber Danielle Windham, Maryam M. Al-Ejji, Noora H. Al-Qahtani, Mohammad K. Hassan, Kenneth A. Mauritz, Randy K. Buchanan, J. Paige Buchanan
Faculty Publications
Fullerene-containing materials have the ability to store and release electrical energy. Therefore, fullerenes may ultimately find use in high-voltage equipment devices or as super capacitors for high electric energy storage due to this ease of manipulating their excellent dielectric properties and their high volume resistivity. A series of structured fullerene (C60) polymer nanocomposites were assembled using the thiol-ene click reaction, between alkyl thiols and allyl functionalized C60 derivatives. The resulting high-density C60-urethane-thiol-ene (C60-Thiol-Ene) networks possessed excellent mechanical properties. These novel networks were characterized using standard techniques, including infrared spectroscopy (FTIR), differential scanning calorimetry …
Advances In Understanding Carboxysome Assembly In Prochlorococcus And Synechococcus Implicate Csos2 As A Critical Component, Fei Cai, Zhicheng Dou, Susan L. Bernstein, Ryan Leverenz, Eric B. Williams, Sabine Heinhorst, Jessup M. Shively, Gordon C. Cannon, Cheryl A. Kerfeld
Advances In Understanding Carboxysome Assembly In Prochlorococcus And Synechococcus Implicate Csos2 As A Critical Component, Fei Cai, Zhicheng Dou, Susan L. Bernstein, Ryan Leverenz, Eric B. Williams, Sabine Heinhorst, Jessup M. Shively, Gordon C. Cannon, Cheryl A. Kerfeld
Faculty Publications
The marine Synechococcus and Prochlorococcus are the numerically dominant cyanobacteria in the ocean and important in global carbon fixation. They have evolved a CO2-concentrating-mechanism, of which the central component is the carboxysome, a self-assembling proteinaceous organelle. Two types of carboxysome, α and β, encapsulating form IA and form IB d-ribulose-1,5-bisphosphate carboxylase/oxygenase, respectively, differ in gene organization and associated proteins. In contrast to the β-carboxysome, the assembly process of the α-carboxysome is enigmatic. Moreover, an absolutely conserved α-carboxysome protein, CsoS2, is of unknown function and has proven recalcitrant to crystallization. Here, we present studies on the CsoS2 protein in …
Efficient Esterification Of Oxidized L-Glutathione And Other Small Peptides, Emily Rose Vogel, William Jackson, Douglas S. Masterson Dr.
Efficient Esterification Of Oxidized L-Glutathione And Other Small Peptides, Emily Rose Vogel, William Jackson, Douglas S. Masterson Dr.
Faculty Publications
xidized l-glutathione was esterified to the tetra methyl ester using thionyl chloride in methanol solvent. Other alcohols were tested and the reaction progress was monitored via ESI-MS. This procedure proved to be compatible with other small peptides not containing serine and cysteine residues. In contrast to previously reported methods this procedure provided convenient access to esterified peptides requiring no purification, extended reaction times, or complicated reaction setups.
A Simple Method For Tuning The Glass Transition Process In Inorganic Phosphate Glasses, René Fulchiron, Imane Belyamani, Joshua U. Otaigbe, Véronique Bounor-Legaré
A Simple Method For Tuning The Glass Transition Process In Inorganic Phosphate Glasses, René Fulchiron, Imane Belyamani, Joshua U. Otaigbe, Véronique Bounor-Legaré
Faculty Publications
The physical modification of glass transition temperature (Tg) and properties of materials via blending is a common practice in industry and academia and has a large economic advantage. In this context, simple production of hitherto unattainable new inorganic glass blends from already existing glass compositions via blending raises much hope with the potential to provide new glasses with new and improved properties, that cannot be achieved with classical glass synthesis, for a plethora of applications such as computers screens, glass-to-metal seals, and storage materials for nuclear wastes. Here, we demonstrate that blends of the specific glass compositions …