Open Access. Powered by Scholars. Published by Universities.®
Physical Sciences and Mathematics Commons™
Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- ADP-glucose pyrophosphorylase (1)
- Absorption spectroscopy (1)
- Adsorption (1)
- Allosteric regulation (1)
- Allosterism (1)
-
- Biological processes (1)
- CO oxidation (1)
- Cadmium (1)
- Catalysts and Catalysis (1)
- Chemical elements (1)
- Chemistry Education (1)
- Dissociation constants (1)
- Drug repurposing (1)
- Engineered heme protein biocatalysts (1)
- Enzyme engineering (1)
- Escherichia coli (1)
- Evolved variants (1)
- Ferritin‐transferrin paradox (1)
- Glucosamine-1-Phosphate (1)
- Glucosamine-6-Phosphate (1)
- Glucose-1-Phosphate (1)
- Glycogenbiosynthesis (1)
- Green Chemistry (1)
- Host proteases (1)
- Hypoxia (1)
- Infrared spectroscopy (1)
- Iron homeostasis (1)
- Lansoprazole (1)
- Lead (1)
- Leishmaniasis (1)
- Publication
- Publication Type
Articles 1 - 13 of 13
Full-Text Articles in Physical Sciences and Mathematics
Velocity Map Images Of Desorbing Oxygen From Sub-Surface States Of Rh(111), Arved C. Dorst, Friedrich Güthoff, Daniel Schauermann, Alec M. Wodtke, Daniel Killelea, Tim Schäfer
Velocity Map Images Of Desorbing Oxygen From Sub-Surface States Of Rh(111), Arved C. Dorst, Friedrich Güthoff, Daniel Schauermann, Alec M. Wodtke, Daniel Killelea, Tim Schäfer
Chemistry: Faculty Publications and Other Works
We combine velocity map imaging (VMI) with temperature-programmed desorption (TPD) experiments to record the angular-resolved velocity distributions of recombinatively-desorbing oxygen from Rh(111). We assign the velocity distributions to desorption from specific surface and sub-surface states by matching the recorded distributions to the desorption temperature. These results provide insight into the recombinative desorption mechanisms and the availability of oxygen for surface-catalyzed reactions.
Cultivating Teacher Expertise In The Landscape Of Green Chemistry: The Development Of Pedagogical Content Knowledge In Beyond Benign’S Lead Teacher Program, Philip Charles Nahlik
Cultivating Teacher Expertise In The Landscape Of Green Chemistry: The Development Of Pedagogical Content Knowledge In Beyond Benign’S Lead Teacher Program, Philip Charles Nahlik
Dissertations
Chemistry Education, Green Chemistry, Pedagogical Content Knowledge, Professional Learning Communities, Sustainability, Teacher Training
Investigation Of Carbon Dioxide Oxidation Reaction Pathways On Rh(111) Via Reflection Absorption Infrared Spectroscopy (Rairs), Elizabeth A. Jamka
Investigation Of Carbon Dioxide Oxidation Reaction Pathways On Rh(111) Via Reflection Absorption Infrared Spectroscopy (Rairs), Elizabeth A. Jamka
Dissertations
CO oxidation, RAIRS, Rh(111), Surface Science, UHV
Site-Directed Mutagenesis Of Serine-72 Reveals The Location Of The Fructose 6-Phosphate Regulatory Site Of The Agrobacterium Tumefaciensadp-Glucose Pyrophosphorylase, Mashael Alghamdi Dr., Rania Ali Z Hussien, Yuanzhang Zheng, Hiral Patel, Matias D. Asencion Diez, Alberto A. Iglesias, Dali Liu, Miguel A. Ballicora
Site-Directed Mutagenesis Of Serine-72 Reveals The Location Of The Fructose 6-Phosphate Regulatory Site Of The Agrobacterium Tumefaciensadp-Glucose Pyrophosphorylase, Mashael Alghamdi Dr., Rania Ali Z Hussien, Yuanzhang Zheng, Hiral Patel, Matias D. Asencion Diez, Alberto A. Iglesias, Dali Liu, Miguel A. Ballicora
Chemistry: Faculty Publications and Other Works
The allosteric regulation of ADP–glucose pyrophosphorylase is critical for the biosynthesis of glycogen in bacteria and starch in plants. The enzyme from Agrobacterium tumefaciens is activated by fructose 6-phosphate (Fru6P) and pyruvate (Pyr). The Pyr site has been recently found, but the site where Fru6P binds has remained unknown. We hypothesize that a sulfate ion previously found in the crystal structure reveals a part of the regulatory site mimicking the presence of the phosphoryl moiety of the activator Fru6P. Ser72 interacts with this sulfate ion and, if the hypothesis is correct, Ser72 would affect the interaction with Fru6P and activation …
Temperature-Resolved Surface Infrared Spectroscopy Of Co On Rh(111) And (2 × 1)-O/Rh(111), Elizabeth A. Jamka, Maxwell Z. Gillum, Christina N. Grytsyshyn-Giger, Faith J. Lewis, Daniel Killelea
Temperature-Resolved Surface Infrared Spectroscopy Of Co On Rh(111) And (2 × 1)-O/Rh(111), Elizabeth A. Jamka, Maxwell Z. Gillum, Christina N. Grytsyshyn-Giger, Faith J. Lewis, Daniel Killelea
Chemistry: Faculty Publications and Other Works
Heterogeneously catalyzed reactions over transition metal surfaces are pillars of chemical industry and account for a significant fraction of the global energy demand. CO oxidation provides insight into the relative reactivity of various oxygenaceous surface phases, and it is necessary to first understand where it binds to the surface and the nature of the local environment to develop robust mechanistic pictures of the reaction. Surface IR spectroscopy is a quantitative technique that also provides information about the binding sites and chemical environments of the adsorbed CO molecules. Here, we report results from a study of CO sticking to clean Rh(111) …
Development Of An Analytical Method For Determination Of Lead And Cadmium In Biological Materials By Gfaas Using Escherichia Coli As Model Substance, Michelle Catherine Gende, Martina Schmeling
Development Of An Analytical Method For Determination Of Lead And Cadmium In Biological Materials By Gfaas Using Escherichia Coli As Model Substance, Michelle Catherine Gende, Martina Schmeling
Chemistry: Faculty Publications and Other Works
In this work, an analytical method was developed for the determination of lead and cadmium in biological samples using graphite furnace atomic absorption spectrometry. Escherichia coli (E. coli) was chosen as model substance for this purpose as it is readily available in most laboratories and can be quickly and easily prepared with a high turnaround rate. Four different sample preparation methods were initially evaluated with respect to percent recovery, limit of detection, and limit of quantification, and the most promising one was developed further. The final process involving microwave assisted digestion of the sample with nitric acid …
Carbohydrate Metabolism In Bacteria: Alternative Specificities In Adp-Glucose Pyrophosphorylases Open Novel Metabolic Scenarios And Biotechnological Tools, Jaina A. Bhayani, Maria Josefina Iglesias, Romina I. Minen, Antonela E. Cereijo, Miguel Ballicora, Alberto A. Iglesias, Matias D. Asencion Diez
Carbohydrate Metabolism In Bacteria: Alternative Specificities In Adp-Glucose Pyrophosphorylases Open Novel Metabolic Scenarios And Biotechnological Tools, Jaina A. Bhayani, Maria Josefina Iglesias, Romina I. Minen, Antonela E. Cereijo, Miguel Ballicora, Alberto A. Iglesias, Matias D. Asencion Diez
Chemistry: Faculty Publications and Other Works
We explored the ability of ADP-glucose pyrophosphorylase (ADP-Glc PPase) from different bacteria to use glucosamine (GlcN) metabolites as a substrate or allosteric effectors. The enzyme from the actinobacteria Kocuria rhizophila exhibited marked and distinctive sensitivity to allosteric activation by GlcN-6P when producing ADP-Glc from glucose-1-phosphate (Glc-1P) and ATP. This behavior is also seen in the enzyme from Rhodococcus spp., the only one known so far to portray this activation. GlcN-6P had a more modest effect on the enzyme from other Actinobacteria (Streptomyces coelicolor), Firmicutes (Ruminococcus albus), and Proteobacteria (Agrobacterium tumefaciens) groups. In addition, we …
Repurposing Lansoprazole And Posaconazole To Treat Leishmaniasis: Integration Of In Vitro Testing, Pharmacological Corroboration, And Mechanisms Of Action, Yash Gupta, Steven Goicoechea, Jesus G. Romero, Raman Mathur, Thomas R. Caulfield, Daniel P. Becker, Ravi Durvasula, Prakasha Kempaiah
Repurposing Lansoprazole And Posaconazole To Treat Leishmaniasis: Integration Of In Vitro Testing, Pharmacological Corroboration, And Mechanisms Of Action, Yash Gupta, Steven Goicoechea, Jesus G. Romero, Raman Mathur, Thomas R. Caulfield, Daniel P. Becker, Ravi Durvasula, Prakasha Kempaiah
Chemistry: Faculty Publications and Other Works
Leishmaniasis remains a serious public health problem in many tropical regions of the world. Among neglected tropical diseases, the mortality rate of leishmaniasis is second only to malaria. All currently approved therapeutics have toxic side effects and face rapidly increasing resistance. To identify existing drugs with antileishmanial activity and predict the mechanism of action, we designed a drug-discovery pipeline utilizing both in-silico and in-vitro methods. First, we screened compounds from the Selleckchem Bio-Active Compound Library containing ~1622 FDA-approved drugs and narrowed these down to 96 candidates based on data mining for possible anti-parasitic properties. Next, we completed preliminary in-vitro testing …
Determination Of Dissociation Constants Of Protein Ligands By Thermal Shift Assay, Jaina A. Bhayani, Miguel Ballicora
Determination Of Dissociation Constants Of Protein Ligands By Thermal Shift Assay, Jaina A. Bhayani, Miguel Ballicora
Chemistry: Faculty Publications and Other Works
The thermal shift assay (TSA) is a powerful tool used to detect molecular interactions between proteins and ligands. Using temperature as a physical denaturant and an extrinsic fluorescent dye, the TSA tracks protein unfolding. This method precisely determines the midpoint of the unfolding transition (Tm" role="presentation">), which can shift upon the addition of a ligand. Though experimental protocols have been well developed, the thermal shift assay data traditionally yielded qualitative results. Quantitative methods for Kd" role="presentation"> determination relied either on empirical and inaccurate usage of Tm" role="presentation"> or on isothermal approaches, which do not take full advantage of the …
Iron Dysregulation In Covid-19 And Reciprocal Evolution Of Sars-Cov-2: Natura Nihil Frustra Facit, Yash Gupta, Dawid Maciorowski, Brian Medernach, Daniel P. Becker Ph.D., Ravi Durvasula, Claudia R. Libertin, Prakasha Kempaiah
Iron Dysregulation In Covid-19 And Reciprocal Evolution Of Sars-Cov-2: Natura Nihil Frustra Facit, Yash Gupta, Dawid Maciorowski, Brian Medernach, Daniel P. Becker Ph.D., Ravi Durvasula, Claudia R. Libertin, Prakasha Kempaiah
Chemistry: Faculty Publications and Other Works
After more than a year of the COVID-19 pandemic, SARS-CoV-2 infection rates with newer variants continue to devastate much of the world. Global healthcare systems are overwhelmed with high positive patient numbers. Silent hypoxia accompanied by rapid deterioration and some cases with septic shock is responsible for COVID-19 mortality in many hospitalized patients. There is an urgent need to further understand the relationships and interplay with human host components during pathogenesis and immune evasion strategies. Currently, acquired immunity through vaccination or prior infection usually provides sufficient protection against the emerging variants of SARS-CoV-2 except Omicron variant requiring recent booster. New …
Rhodium-Catalyzed Decarbonylation Of Aroyl Chlorides, Wiktoria M. Koza
Rhodium-Catalyzed Decarbonylation Of Aroyl Chlorides, Wiktoria M. Koza
Dissertations
The development of efficient strategies for the synthesis of aryl–halogen bonds is highly desirable due to the prevalence of these moieties in pharmaceuticals, agrochemicals, and organic synthesis. Although there are numerous applications of aryl chlorides in chemistry, an efficient strategy for the preparation of these molecules is underdeveloped. Transition metal-catalyzed decarbonylation provides an efficient and selective approach for aryl–halogen bond formation. There has been significant progress in the development of new decarbonylation strategies, particularly involving aldehydes for the synthesis of new carbon–hydrogen (C–H) bonds or for cross-coupling reactions. However, transition metal-catalyzed decarbonylation methods for carbon–halogen (C–X) bond formation have been …
Biocatalytic Intramolecular C−H Aminations Via Engineered Heme Proteins: Full Reaction Pathways And Axial Ligand Effects, Yang Wei, Melissa Conklin, Yong Zhang
Biocatalytic Intramolecular C−H Aminations Via Engineered Heme Proteins: Full Reaction Pathways And Axial Ligand Effects, Yang Wei, Melissa Conklin, Yong Zhang
Chemistry: Faculty Publications and Other Works
Engineered heme protein biocatalysts provide an efficient and sustainable approach to develop amine-containing compounds through C−H amination. A quantum chemical study to reveal the complete heme catalyzed intramolecular C−H amination pathway and protein axial ligand effect was reported, using reactions of an experimentally used arylsulfonylazide with hemes containing L=none, SH−, MeO−, and MeOH to simulate no axial ligand, negatively charged Cys and Ser ligands, and a neutral ligand for comparison. Nitrene formation was found as the overall rate-determining step (RDS) and the catalyst with Ser ligand has the best reactivity, consistent with experimental reports. Both RDS and non-RDS (nitrene transfer) …
Synthesis And Characterization Of Ruthenium Complexes And Their Potential And Photosensitizers For Photodynamic Therapy, Keit Dine
Master's Theses
Cancer is one of the leading causes of death in the world with over 10 million people dying of the disease every year. Inorganic complexes supported by platinum metal centers are often used in cancer treatment. However, these complexes lack selectivity for tumor cells. As such, other alternatives to platinum have been explored. Ruthenium (Ru) complexes are one of the most promising candidates due to their ability to be used as photosensitizing drugs in photodynamic therapy. Herein, we report the design, synthesis, and characterization of two novel ruthenium-based complexes. One compound is a Ru(II) homoleptic complex, while the other is …