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- Cucurbit[8]uril (3)
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Articles 1 - 5 of 5
Full-Text Articles in Physical Sciences and Mathematics
Visualising Dna: Footprinting And 1-2d Gels, Adam Urbach, Michael Waring
Visualising Dna: Footprinting And 1-2d Gels, Adam Urbach, Michael Waring
Adam R Urbach
The study of molecular recognition of DNA by natural and synthetic ligands has made enormous progress due in large part to the discovery and development of methods for separating DNA fragments by gel electrophoresis in one and two dimensions, and for characterizing DNA–ligand complexes by footprinting techniques.
Scope Of Amino Acid Recognition By Cucurbit[8]Uril, Preetika Rajgariah, Adam Urbach
Scope Of Amino Acid Recognition By Cucurbit[8]Uril, Preetika Rajgariah, Adam Urbach
Adam R Urbach
This paper describes the molecular recognition of amino acids by cucurbit[8]uril (Q8) and by the 1:1 complex between Q8 and methyl viologen (MV) in purely aqueous solution. These hosts are known to bind aromatic peptides with high affinity and sequence specificity, but prior work has focused on only a small subset of amino acids. In an effort to elucidate the scope and limitations of amino acid recognition by Q8 and Q8•MV, a comprehensive examination of the 20 genetically encoded amino acids was carried out by 1H NMR spectroscopy and isothermal titration calorimetry. We find that both Q8 and Q8•MV …
Dna Complexes: Durable Binders, Adam Urbach
Dna Complexes: Durable Binders, Adam Urbach
Adam R Urbach
The ability to program molecules to bind to specific sequences of DNA with high fidelity and stability is an important goal of chemical biology. Such molecules can compete with transcription-factor proteins and thus modulate the transcription of genes. They are therefore promising for both elucidating the complex regulation of mammalian gene expression, and developing pharmaceuticals and medical diagnostics targeted to genetic abberations. Writing in Nature Chemistry, Brent Iverson and co-workers have now described the excellent sequence specificity and unusual binding kinetics of a stable DNA–ligand complex, which exhibits a half-life of 16 days.
Effects Of The Number And Placement Of Positive Charges On Viologen–Cucurbit[N]Uril Interactions, Gretchen Vincil, Adam Urbach
Effects Of The Number And Placement Of Positive Charges On Viologen–Cucurbit[N]Uril Interactions, Gretchen Vincil, Adam Urbach
Adam R Urbach
Recent developments in the synthesis and applications of the cucurbit[n]uril family of synthetic hosts has led to an increasing interest in the detailed studies of their interactions with a wide variety of guests. This paper describes a quantitative study of the effects of the number and placement of positive charges on the binding of viologen guests to cucurbit[7]uril and cucurbit[8]uril. A series of viologen derivatives with one to four charges was characterised by isothermal titration calorimetry, 1H NMR spectroscopy and mass spectrometry to determine the stoichiometry, affinity and mode of binding. These data show that stoichiometry can be controlled by …
Benzobis(Imidazolium)–Cucurbit[8]Uril Complexes For Binding And Sensing Aromatic Compounds In Aqueous Solution, Frank Biedermann, Urs Rauwald, Monika Cziferszky, Kyle Williams, Lauren Gann, Bi Guo, Adam Urbach, Christopher Bielawski, Oren Scherman
Benzobis(Imidazolium)–Cucurbit[8]Uril Complexes For Binding And Sensing Aromatic Compounds In Aqueous Solution, Frank Biedermann, Urs Rauwald, Monika Cziferszky, Kyle Williams, Lauren Gann, Bi Guo, Adam Urbach, Christopher Bielawski, Oren Scherman
Adam R Urbach
The utilities of benzobis(imidazolium) salts (BBIs) as stable and fluorescent components of supramolecular assemblies involving the macrocyclic host, cucurbit[8]uril (CB[8]), are described. CB[8] has the unusual ability to bind tightly and selectively to two different guests in aqueous media, typically methyl viologen (MV) as the first guest, followed by an indole, naphthalene, or catechol-containing second guest. Based on similar size, shape, and charge, tetramethyl benzobis(imidazolium) (MBBI) was identified as a potential alternative to MV that would increase the repertoire of guests for cucurbit[8]uril. Isothermal titration calorimetry (ITC) studies showed that MBBI binds to CB[8] in a 1:1 ratio with an …