Open Access. Powered by Scholars. Published by Universities.®
Physical Sciences and Mathematics Commons™
Open Access. Powered by Scholars. Published by Universities.®
- Publication
Articles 1 - 3 of 3
Full-Text Articles in Physical Sciences and Mathematics
Circular Dichroism Spectroscopy In The Undergraduate Curriculum, Adam Urbach
Circular Dichroism Spectroscopy In The Undergraduate Curriculum, Adam Urbach
Adam R Urbach
Circular dichroism spectropolarimetry (CD) is a method of optical spectroscopy that seems in most practical ways like UV−visible spectroscopy. The main difference between the two methods is that CD, instead of measuring the absorbance of light as a function of wavelength, measures the difference in absorbance of left versus right circularly polarized light as a function of wavelength. A CD spectrum is an observation of the structure of a chiral compound; it often serves as a “fingerprint” of the structure of biological molecules such as proteins and nucleic acids. For this reason, CD has been broadly applied in biochemistry and …
Complementary Spectroscopic Assays For Investigating Protein−Ligand Binding Activity: A Project For The Advanced Chemistry Laboratory, David Mascotti, Mark Waner
Complementary Spectroscopic Assays For Investigating Protein−Ligand Binding Activity: A Project For The Advanced Chemistry Laboratory, David Mascotti, Mark Waner
Mark J. Waner
A protein−ligand binding, guided-inquiry laboratory project with potential application across the advanced undergraduate curriculum is described. At the heart of the project are fluorescence and spectrophotometric assays utilizing biotin-4-fluorescein and streptavidin. The use of the same stock solutions for an assay that may be examined by two distinct spectroscopic techniques offers an opportunity to discuss not only protein−ligand binding but also to compare instrumental techniques and to discuss the underlying physical principles that are the origins of the assays. In addition to critically evaluating analytical techniques, students are pushed to develop quantitative pipetting, estimation, and experimental-design skills to collect and …
Complementary Spectroscopic Assays For Investigating Protein−Ligand Binding Activity: A Project For The Advanced Chemistry Laboratory, David Mascotti, Mark Waner
Complementary Spectroscopic Assays For Investigating Protein−Ligand Binding Activity: A Project For The Advanced Chemistry Laboratory, David Mascotti, Mark Waner
David P. Mascotti
A protein−ligand binding, guided-inquiry laboratory project with potential application across the advanced undergraduate curriculum is described. At the heart of the project are fluorescence and spectrophotometric assays utilizing biotin-4-fluorescein and streptavidin. The use of the same stock solutions for an assay that may be examined by two distinct spectroscopic techniques offers an opportunity to discuss not only protein−ligand binding but also to compare instrumental techniques and to discuss the underlying physical principles that are the origins of the assays. In addition to critically evaluating analytical techniques, students are pushed to develop quantitative pipetting, estimation, and experimental-design skills to collect and …