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Full-Text Articles in Physical Sciences and Mathematics
Simultaneous Determination Of Glucose And L‐Glutamate Using A Capillary Enzyme Reactor With Electrochemical Detection, Stephanie Hooper, Mark Anderson
Simultaneous Determination Of Glucose And L‐Glutamate Using A Capillary Enzyme Reactor With Electrochemical Detection, Stephanie Hooper, Mark Anderson
Mark R. Anderson
An electrophoresis capillary that incorporates two enzymes for the simultaneous determination of glucose and l-glutamate is described. The enzymes deposited along the separation capillary walls react with their respective substrate as they are separated during the electrophoresis to produce hydrogen peroxide that is detected by amperometry as the hydrogen peroxide zone emerges from the end of the capillary. Even though both enzyme reactions produce a common product, hydrogen peroxide, the hydrogen peroxide produced by each enzyme reaction stays in narrow zones that migrate the length of the capillary at different rates. The rate of migration for the individual H2O2 zones …
Application Of A Square‐Wave Potential Program For Time‐Dependent Amperometric Detection In Capillary Electrophoresis, Stephanie Hooper, David Roach, Mark Anderson
Application Of A Square‐Wave Potential Program For Time‐Dependent Amperometric Detection In Capillary Electrophoresis, Stephanie Hooper, David Roach, Mark Anderson
Mark R. Anderson
Use of a square-wave potential program for time-dependent amperometric detection of analyte zones in capillary electrophoresis (CE) is described. Electrochemical detection for CE requires that the separation field be isolated from that of the electrochemical detection. This is generally done by physically separating the CE separation field from that of the detection. By applying a time variant potential program to the detection electrode, the detector current has a time dependence that can be used to help isolate the electrochemical detection current from that of the separation. When using a 20 μm inner-diameter capillary, we find that a square-wave potential program …
Modification Of A Capillary For Electrophoresis By Electrostatic Self-Assembly Of An Enzyme For Selective Determination Of The Enzyme Substrate, Stephanie Hooper, Mark Anderson
Modification Of A Capillary For Electrophoresis By Electrostatic Self-Assembly Of An Enzyme For Selective Determination Of The Enzyme Substrate, Stephanie Hooper, Mark Anderson
Mark R. Anderson
The integration of a separation capillary for capillary electrophoresis (CE) with an on-column enzyme reaction for selective determination of the enzyme substrate is described. Enzyme immobilization is achieved by electrostatic assembly of poly(diallydimethylammonium chloride) (PDDA) followed by adsorption of a mixture of the negatively charged enzyme glucose oxidase (GOx) and anionic poly(styrenesulfonate) (PSS). The reaction of glucose with the GOx produces hydrogen peroxide which migrates the length of the capillary and is detected amperometrically at the capillary outlet. The enzyme reaction occurs during a capillary separation, allowing selective determination of the substrate in complex samples without the need for pre- …