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Articles 1 - 10 of 10
Full-Text Articles in Physical Sciences and Mathematics
Modeling In Microbial Batch Culture And Its Parameter Identification, Zhaohua Gong, Chongyang Liu, Enmin Feng
Modeling In Microbial Batch Culture And Its Parameter Identification, Zhaohua Gong, Chongyang Liu, Enmin Feng
Chongyang Liu
In this paper, the nonlinear dynamical system of batch fermentation is investigated in the bioconversion of glycerol to 1,3-propanediol(1,3-PD) by Klebsiella pneumoniae. Taking account of the kinetic behavior and experimental results in the batch cultures, we propose a two-stage dynamical system to formulate the fermentation process. Then some properties of the proposed system are proved. In view of the big errors between observations and numerical simulation results, we subsequently establish a parameter identification model to identify parameters in the system. The identifiability of the model is also discussed. Finally, in order to find the optimal parameters of the identification model, …
Computational Method For Inferring Objective Function Of Glycerol Metabolism In Klebsiella Pneumoniae, Zhaohua Gong, Chongyang Liu, Enmin Feng, Qingrui Zhang
Computational Method For Inferring Objective Function Of Glycerol Metabolism In Klebsiella Pneumoniae, Zhaohua Gong, Chongyang Liu, Enmin Feng, Qingrui Zhang
Chongyang Liu
Flux balance analysis (FBA) is an effective tool in the analysis of metabolic network. It can predict the flux distribution of engineered cells, whereas the accurate prediction depends on the reasonable objective function. In this work, we propose two nonlinear bilevel programming models on anaerobic glycerol metabolism in Klebsiella pneumoniae (K. pneumoniae) for 1,3-propanediol (1,3-PD) production. One intends to infer the metabolic objective function, and the other is to analyze the robustness of the objective function. In view of the models’ characteristic an improved genetic algorithm is constructed to solve them, where some techniques are adopted to guarantee all chromosomes …
Modelling And Optimal Control For Nonlinear Multistage Dynamical System Of Microbial Fed-Batch Culture, Chongyang Liu, Zhaohua Gong, Enmin Feng, Hongchao Yin
Modelling And Optimal Control For Nonlinear Multistage Dynamical System Of Microbial Fed-Batch Culture, Chongyang Liu, Zhaohua Gong, Enmin Feng, Hongchao Yin
Chongyang Liu
In this paper, we propose a new controlled multistage system to formulate the fed-batch culture process of glycerol bio-dissimilation to 1,3-propanediol (1,3-PD) by regarding the feeding rate of glycerol as a control function. Compared with the previous systems, this system doesn’t take the feeding process as an impulsive form, but a time-continuous process, which is much closer to the actual culture process. Some properties of the above dynamical system are then proved. To maximize the concentration of 1,3-PD at the terminal time, we develop an optimal control model subject to our proposed controlled multistage system and continuous state inequality constraints. …
Optimal Control And Properties Of Nonlinear Multistage Dynamical System For Planning Horizontal Well Paths, Zhaohua Gong, Chongyang Liu, Enmin Feng
Optimal Control And Properties Of Nonlinear Multistage Dynamical System For Planning Horizontal Well Paths, Zhaohua Gong, Chongyang Liu, Enmin Feng
Chongyang Liu
The goal of planning a horizontal well path is to obtain a trajectory that arrives at a given target subject to various constraints. In this paper, the optimal control problem subject to a nonlinear multistage dynamical system (NMDS) for horizontal well paths is investigated. Some properties of the multistage system are proved. In order to derive the optimality conditions, we transform the optimal control problem into one with control constraints and inequality-constrained trajectories by defining some functions. The properties of these functions are then discussed and optimality conditions for optimal control problem are also given. Finally, an improved simplex method …
Optimal Control For Nonlinear Dynamical System Of Microbial Fed-Batch Culture, Chongyang Liu
Optimal Control For Nonlinear Dynamical System Of Microbial Fed-Batch Culture, Chongyang Liu
Chongyang Liu
In fed-batch culture of glycerol bio-dissimilation to 1, 3-propanediol (1, 3-PD), the aim of adding glycerol is to obtain as much 1, 3-PD as possible. So a proper feeding rate is required during the process. Taking the concentration of 1, 3-PD at the terminal time as the performance index and the feeding rate of glycerol as the control function, we propose an optimal control model subject to a nonlinear dynamical system and constraints of continuous state and non-stationary control. A computational approach is constructed to seek the solution of the above model in two aspects. On the one hand we …
A Quantitative Taqman Mgb Real-Time Polymerase Chain Reaction Based Assay For Detection Of The Causative Agent Of Crayfish Plague Aphanomyces Astaci, Torstein Tengs
Dr. Torstein Tengs
Here we present the development and first validation of a TaqMan minor groove binder (MGB) real-time polymerase chain reaction (RT-PCR) method for quantitative and highly specific detection of Aphanomyces astaci, the causative agent of crayfish plague. The assay specificity was experimentally assessed by testing against DNA representative of closely related oomycetes, and theoretically assessed by additional sequence similarity analyses comparing the primers and probe sequences to available sequences in EMBL/GenBank. The target of the assay is a 59 bp unique sequence motif of A. astaci found in the internal transcribed spacer 1 of the nuclear ribosomal gene cluster. A standard …
Quantifying Total And Sustainable Agricultural Biomass Resources In South Dakota—A Preliminary Assessment, Kurt A. Rosentrater, Dennis Todey, Russell Persyn
Quantifying Total And Sustainable Agricultural Biomass Resources In South Dakota—A Preliminary Assessment, Kurt A. Rosentrater, Dennis Todey, Russell Persyn
Kurt A. Rosentrater
Conversion of biomass is considered the next major advance in biorenewable fuels, energy, and products. Wholesale conversion to biomass utilization could result in removal of current crop residues from agricultural fields (on prime agricultural lands) or even implementation of different crops and cropping strategies (i.e., switchgrass on marginal lands). To date, the driver for biomass processing has been economics and limitations on the conversion of the lignocellulose. Over the last forty years significant investments and resultant changes in management practices in the agricultural sector have focused on soil and water conservation. One of the major efforts has focused on conservation-till …
Properties Of Solvent Extracted Low-Oil Corn Distillers Dried Grains With Solubles, Kurt A. Rosentrater, Jennifer A. Saunders
Properties Of Solvent Extracted Low-Oil Corn Distillers Dried Grains With Solubles, Kurt A. Rosentrater, Jennifer A. Saunders
Kurt A. Rosentrater
Corn-based ethanol is exponentially growing in the US, as is the need for valuable uses for coproducts of the production process, such as distillers dried grains with solubles (DDGS). Currently, DDGS is used as livestock feed, thereby replacing some corn components in animal diets. As the industry continues to grow, there will be an increased need to find additional uses for DDGS. Physical and chemical properties of coproduct streams are becoming increasingly investigated, as these characteristics affect many aspects of utilization, such as target species, optimal dietary substitution rates, transportation, flowability, and behavior during storage. Potential avenues for future use …
Design Properties For Molded, Corn-Based Ddgs-Filled Phenolic Resin, R. A. Tatara, Kurt A. Rosentrater, S. Suraparaju
Design Properties For Molded, Corn-Based Ddgs-Filled Phenolic Resin, R. A. Tatara, Kurt A. Rosentrater, S. Suraparaju
Kurt A. Rosentrater
With the rapid growth in the ethanol fuel industry in recent years, considerable research is being devoted to maximizing the use of processing coproducts, such as distillers dried grains with solubles (DDGS), typically for livestock diets. Because these residues contain high fiber levels, they may be amendable to incorporation into polymers as well, which is an option that could garner greater economic returns. Thus, the goal of this study was to demonstrate the viability of using corn-based DDGS as a biofiller with phenolic resin, in order to produce a novel biomaterial. DDGS was blended with phenolic resin at four levels …
Review Of Biocd Physics And Technology, David D. Nolte
Review Of Biocd Physics And Technology, David D. Nolte
David D Nolte
Spinning biodisks have advantages that make them attractive for specialized biochip applications. The two main classes of spinning biodisks are microfluidic disks and bio-optical compact disks BioCD. Microfluidic biodisks take advantage of noninertial pumping for lab-on-a-chip devices using noninertial valves and switches under centrifugal and Coriolis forces to distribute fluids about the disks. BioCDs use spinning-disk interferometry, under the condition of common-path phase quadrature, to perform interferometric label-free detection of molecular recognition and binding. The optical detection of bound molecules on a disk is facilitated by rapid spinning that enables high-speed repetitive sampling to eliminate 1/ f noise through common-mode …