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Biological and Chemical Physics

Doctoral Dissertations

2016

Articles 1 - 6 of 6

Full-Text Articles in Physical Sciences and Mathematics

Study Of Charge Transport Mechanism In Microbial Nanowires, Ramesh Adhikari Nov 2016

Study Of Charge Transport Mechanism In Microbial Nanowires, Ramesh Adhikari

Doctoral Dissertations

Conductivity of an individual proteinaceous filaments, called pili or microbial nanowires, produced by wild type Geobacter sulfurreducens was measured using a low-noise electrical transport technique. It was shown that the conductivity is diminished if aromatic amino acid residues are removed from the pili. It was demonstrated that the conductivity of G. sulfurreducens pili is similar to that of synthetic organic polymers and is related to the respiration rate of the bacteria. Conductivity measurements were performed on microbial nanowires produced by other species of Geobacter with a different aromatic amino acid distribution along the wire. Also, measurements on a mutated pili …

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A Quantitative Measurement Of Structural Changes Of Rna Kissing Complexes Using Fluorescence Resonance Energy Transfer, Sheema Rahmanseresht Nov 2016

A Quantitative Measurement Of Structural Changes Of Rna Kissing Complexes Using Fluorescence Resonance Energy Transfer, Sheema Rahmanseresht

Doctoral Dissertations

Many RNA interactions in cells occur in the form of loop-loop interactions, also known as a "kissing complex". In the bacterial and viral systems discussed here, there are transiently bound proteins involved that modulate the function of kissing complex. These proteins either stabilize the kissing complex or facilitate its conversion to extended duplex. I studied R1inv-R2inv kissing complex (KC), derivatived from RNAI-RNAII complex of E.Coli. Rop protein is known to stabilize the bent R1inv-R2inv KC against dissociation. The goal was to study structural change of this kissing complex after binding of the stabilizing Rop protein. In this work for …

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Morphological And Material Effects In Van Der Waals Interactions, Jaime C. Hopkins Jul 2016

Morphological And Material Effects In Van Der Waals Interactions, Jaime C. Hopkins

Doctoral Dissertations

Van der Waals (vdW) interactions influence a variety of mesoscale phenomena, such as surface adhesion, friction, and colloid stability, and play increasingly important roles as science seeks to design systems on increasingly smaller length scales. Using the full Lifshitz continuum formulation, this thesis investigates the effects of system materials, shapes, and configurations and presents open-source software to accurately calculate vdW interactions. In the Lifshitz formulation, the microscopic composition of a material is represented by its bulk dielectric response. Small changes in a dielectric response can result in substantial variations in the strength of vdW interactions. However, the relationship between these …

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Synthesis And Characterization Of Polymeric Anion Exchange Membranes, Wenxu Zhang Jul 2016

Synthesis And Characterization Of Polymeric Anion Exchange Membranes, Wenxu Zhang

Doctoral Dissertations

As alkaline anion exchange membrane fuel cells (AAEMFC) are regarded as promising and important energy devices, the development of high performance anion exchange membranes are in urgent need, as well as fundamental investigation on the structure-property relationship, which are the motivation of this dissertation. Three different polymer systems are presented and focused on polymer synthesis, material morphology, and ion transport phenomena. Crosslinked membranes are promising as practical materials, however, the understanding and further improvement of its performance is hindered by the lack of an ordered morphology or well-defined chemical structure. In Chapter 2, a series of crosslinked membranes were design …

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Biophysical Studies Of Cell Division Protein Localization Mechanisms In Escherichia Coli, Matthew Wayne Bailey May 2016

Biophysical Studies Of Cell Division Protein Localization Mechanisms In Escherichia Coli, Matthew Wayne Bailey

Doctoral Dissertations

How nanometer-scale proteins position accurately within micron-scale bacteria has intrigued both biologists and physicists alike. A critical process requiring precise protein localization is cell division. In most bacteria, cell division starts with the self-assembly of the FtsZ proteins into filaments that form a ring-like structure encircling the cell at its middle, the Z-ring. The Z-ring is a scaffold for additional proteins that synthesize the lateral cell wall which separates the two daughter cells. If division planes are misplaced relative to bacterial chromosomes, also called nucleoids, daughter cells with incomplete genetic material can be produced. In Escherichia coli, research carried out …

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Voltage Driven Translocation Of Polyelectrolytes Through Nanopores, Byoung-Jin Jeon Mar 2016

Voltage Driven Translocation Of Polyelectrolytes Through Nanopores, Byoung-Jin Jeon

Doctoral Dissertations

Recently, translocations of polyelectrolyte molecules through membrane channel protein pores or solid-state nanopores have been actively studied. Although the polymer translocation researches emerged mainly due to technological demands in terms of genome sequencing, the detailed physics of the single molecule transport through a narrow channel remains fully understood. To obtain further understanding of common features of the translocation process, this thesis focuses on the effects of salt concentration, pore-polymer electrostatic interactions, and externally applied electric field on the voltage-driven polymer translocations. The study is carried out by performing a series of systematically designed experiments using alpha-hemolysin (αHL) protein pore to …

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