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Full-Text Articles in Physical Sciences and Mathematics
The Role Of Hsp70 In Cancer: A Study Of The Hsp70 / Akt Relationship, John Koren
The Role Of Hsp70 In Cancer: A Study Of The Hsp70 / Akt Relationship, John Koren
USF Tampa Graduate Theses and Dissertations
The Hsp70 family of molecular chaperones is essential for
protein folding, re-folding misfolded client proteins, clearance
of aberrant client proteins, and can also inhibit programmed
cell death. There are two major cytosolic members of this
family: the constitutive Hsc70, and the inducible Hsp72. Under
stress conditions the Hsp70 family protects the cell from
protein related damage by the induction of Hsp72. Hsc70 and
Hsp72 are highly homologous with minor differences in
substrate binding. In cancers, Hsp72 is commonly induced and
this induction is thought to aid in cancer cell survival. In these
studies we demonstrate the differential regulation of the …
Protein Profiling Of Adenine Nucleoside And Nucleotide Analogs Binding Proteins Using N6-Biotinylated-8-Azidoadenosine Analogs As Affinity Based Protein Profiling Probes, Shikha Mahajan
USF Tampa Graduate Theses and Dissertations
Identification of differential expressions of proteins in proteomic profiles of biological samples shows great potential as a valuable technique for the early diagnosis of various diseases. An important challenge in modern protein profiling approaches is to reduce the complexity of the samples by limiting the number of proteins that need to be evaluated for distinction in the expression between normal and deceased cells. In this research, an affinity based approach for the enrichment of nucleotide and nucleoside binding proteins from a complex cell proteome has been developed. To achieve this goal, new N6-biotinylated-8-azido-adenosine probes (AdoRs) have been designed and synthesized …