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Biochemistry

Dissertations, Theses, and Masters Projects

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Articles 1 - 16 of 16

Full-Text Articles in Physical Sciences and Mathematics

A Mathematical Model Of Tau Protein Hyperphosphorylation: The Effects Of Kinase Inhibitors As A Theoretical Alzheimer's Disease Therapy, Patrick Neil Blank Jan 2015

A Mathematical Model Of Tau Protein Hyperphosphorylation: The Effects Of Kinase Inhibitors As A Theoretical Alzheimer's Disease Therapy, Patrick Neil Blank

Dissertations, Theses, and Masters Projects

No abstract provided.


Thermochemistry Of Amino Acids And Constrained Diamines, Erica Jane Tullo Jan 2012

Thermochemistry Of Amino Acids And Constrained Diamines, Erica Jane Tullo

Dissertations, Theses, and Masters Projects

The gas-phase proton affinities of several highly basic amino acids and diamines were determined using the extended kinetic method in an ESI-quadrupole ion trap instrument. The non-protein amino acid L-canavanine is structurally related to L-arginine with an oxygen substituted for the terminal methylene group of L-arginine and is highly toxic to humans. The proton affinity of L-arginine, a protein amino acid, was determined to be 1036 kJ mol -1, whereas the proton affinity of L-canavanine was determined to be 1005 kJ mol-1. Thus, substitution of an oxyguanidino group for the guanidine group in L-arginine results in a large decrease in …


The Role Of Copepods And Heterotrophic Dinoflagellates In The Production Of Dissolved Organic Matter And Inorganic Nutrients, Grace Kathleen Saba Jan 2010

The Role Of Copepods And Heterotrophic Dinoflagellates In The Production Of Dissolved Organic Matter And Inorganic Nutrients, Grace Kathleen Saba

Dissertations, Theses, and Masters Projects

Zooplankton play a key role in the cycling of dissolved organic matter (DOM) and inorganic nutrients. The factors that affect these processes, however, are not fully understood. I measured the effects of various diets on DOM and inorganic nutrient production by the copepod Acartia tonsa and the heterotrophic dinoflagellate Oxyrrhis marina, and explored the mechanisms of nutrient release from copepods. Copepods feeding on a mixed diet, the preferred diet of most copepods, had significantly lower dissolved organic carbon (DOC), ammonium (NH4+), and total dissolved nitrogen (TDN) release rates compared to feeding on a carnivorous or herbivorous diet. Thus, copepod feeding …


Identification And Quantification Of Polycyclic Musks And Methyl-Triclosan In Unknown Freshwater Fish Tissue Samples, Elizabeth Mara Jacobs Jan 2009

Identification And Quantification Of Polycyclic Musks And Methyl-Triclosan In Unknown Freshwater Fish Tissue Samples, Elizabeth Mara Jacobs

Dissertations, Theses, and Masters Projects

No abstract provided.


Environmental Stress In Hard Coral: Evaluating Lipid As An Indicator Of Sublethal Stress On Short Time Scales, David Harold Niebuhr Jan 1999

Environmental Stress In Hard Coral: Evaluating Lipid As An Indicator Of Sublethal Stress On Short Time Scales, David Harold Niebuhr

Dissertations, Theses, and Masters Projects

Lipid quality was evaluated in Montastrea spp. under sediment- and heat-stressed conditions to evaluate lipid ratio as an indicator of sub-lethal stress on short time scales. The ratio of storage lipid (wax ester + triacylglyceride) to structural lipid (sterol esters + phospholipid) decreased significantly (0.25 to 0.14, p < 0.01) after experimental sedimentation. FAME analysis of colonies exposed to experimental sedimentation showed a reduction of the algal, 18:3(n-6) and 18:4(n-3), polyunsaturated fatty acid (PUFA) in the polar lipid fraction. This loss of PUFA suggests a loss of algal membrane in sediment-stressed colonies. Lipid quality was similarly measured in M. faveolata over a 10-day period. Mean (n = 20) ratio of storage to structural lipid in M. faveolata dropped from 2.43 to a level of 0.98 immediately following a natural sedimentation event before recovering to levels of 1.4 and 2.9 on post-storm days 2 and 4, respectively. Colonies of M. annularis subject to heat-stress (35??C) exhibited no significant change in storage lipid ratio, while levels of Free Fatty Acids increased significantly from 0.012 (n = 22) to 0.156 mg lipid/g dry tissue (n = 22)(p < 0.05). FAME analysis of tissue lipids extracted from the heat-stressed colonies showed changes in the polar fraction, with significant decreases in the 18:3(n-6), 18:3(n-3), 18:4(n-3), 20:4(n-6) and 20:5(n-3) (p < 0.05) PUFA and subsequent significant increases in the saturated fatty acids, 16:0 and 18:0 (p < 0.05). These changes in lipid quantity and quality indicate possible oxidation and preferential digestion of zooxanthellar membranes. Stress experiments were repeated in M. annularis using VacutainerRTM blood collection tubes to collect micro-tissue samples without destroying skeleton of the sample colonies. A significant decrease in storage: structural lipid ratio after sedimentation was also detected using the micro-tissue technique. This study indicates that the relative abundance of lipid subclass components can indicate sub-lethal environmental stress, on short time scales, in M. annularis and M. faveolata. Furthermore, micro-tissue collection techniques permit repeated monitoring coral colonies to assess the manifestation of stress from first detection of impact at the cellular level to changes in community to changes in community structure detectable over longer time scales.


Determination Of Trace Levels Of Lead In Whole Blood By Graphite Furnace Atomic Absorption Spectroscopy, Leslie A. Sombers Jan 1998

Determination Of Trace Levels Of Lead In Whole Blood By Graphite Furnace Atomic Absorption Spectroscopy, Leslie A. Sombers

Dissertations, Theses, and Masters Projects

No abstract provided.


Perkinsus Marinus Extracellular Proteases: Modulation Of Production By Environmental Factors And Effects On The Host Defense Parameters Of The Eastern Oyster Crassostrea Virginica, Kathleen A. Garreis Jan 1996

Perkinsus Marinus Extracellular Proteases: Modulation Of Production By Environmental Factors And Effects On The Host Defense Parameters Of The Eastern Oyster Crassostrea Virginica, Kathleen A. Garreis

Dissertations, Theses, and Masters Projects

No abstract provided.


Nutrition And Feeding In Striped Bass Morone Saxatilis Larvae: Lipid And Fatty Acid Requirements And Microencapsulated Diets, Sureyya Ozkizilcik Jan 1995

Nutrition And Feeding In Striped Bass Morone Saxatilis Larvae: Lipid And Fatty Acid Requirements And Microencapsulated Diets, Sureyya Ozkizilcik

Dissertations, Theses, and Masters Projects

There has been a growing interest in the commercial aquaculture of striped bass, Morone saxatilis. Little is known, however, about the nutritional requirements and feeding physiology of striped bass larvae. The objectives of this study were (a) to evaluate the efficiency of the live food enrichment techniques in studying the nutritional requirements for polyunsaturated fatty acids (PUFA), phospholipids and free amino acids, (b) to determine the ontogenetic changes of lipolytic enzymes, and (c) to develop a microencapsulated diet for striped bass larvae as full or partial replacement of live food. Three enrichment techniques, namely, microencapsulated fish oil, yeast/fish oil emulsion …


Evaluation Of A Technique To Quantify Lipids In Bivalve Larvae Crassostrea Virginica Gmelin, Using Nile Red And Epiflourescence Microscopy, Laura Liliana Castell Perez Jan 1991

Evaluation Of A Technique To Quantify Lipids In Bivalve Larvae Crassostrea Virginica Gmelin, Using Nile Red And Epiflourescence Microscopy, Laura Liliana Castell Perez

Dissertations, Theses, and Masters Projects

No abstract provided.


Monooxygenase Induction And Lethality As Endpoints In Aquatic Toxicology, Daniel W. Sved Jan 1991

Monooxygenase Induction And Lethality As Endpoints In Aquatic Toxicology, Daniel W. Sved

Dissertations, Theses, and Masters Projects

Spot, Leiostomus xanthurus, were exposed to suspended sediments (&\approx&20 mg/L) contaminated with polycyclic aromatic hydrocarbons (PAH) in a laboratory flow-through system to evaluate the applicability of hepatic ethoxyresorufin O-deethylase (EROD) induction as an indicator of PAH exposure. PAH sources tested were coal-tar creosote (CTC), a low molecular weight fraction of creosote (LMWF), and a high molecular weight fraction of creosote (HMWF). A standard 96-h acute toxicity test was conducted to ensure that PAH concentrations tested in induction studies were sub-acutely toxic. The 96-h LC50 for spot was 1740 &\mu&g PAH/L (95% confidence interval = 1480-2060 &\mu&g PAH/L). The lowest concentration …


Effects Of Selected Nitrogen-Containing Aromatic Compounds (Ncacs) On Physiological Properties In Escherichia Coli, W. James Catallo Iii Jan 1989

Effects Of Selected Nitrogen-Containing Aromatic Compounds (Ncacs) On Physiological Properties In Escherichia Coli, W. James Catallo Iii

Dissertations, Theses, and Masters Projects

This research examined the effects of quinoline and 4-azafluorene on respiratory electron transport rate (ET), outer membrane permeability and topology, oxygen consumption, and viable cell density in Escherichia coli cell suspensions. ET was estimated spectrophotometrically using INT (2-(p-iodophenyl)-3-(p-nitrophenyl)-5-(phenyl)-2H-tetrazolium chloride, which is reduced in vivo to a red colored formazan (INTF). Both test compounds caused anomalous dose-response behavior in INT assays: in a defined window of doses, ET rates near or above the controls were observed. These doses showed altered INT reduction kinetics, decreased cellular oxygen demand, and decreased viable cell densities. Experiments with E. coli spheroplast preparations, gram(+) cells, and …


Synthesis And Characterization Of Some Oxoperoxopolycarboxylato Vanadates (V) And Their Biochemical Significance, Myunghi Lee Jan 1984

Synthesis And Characterization Of Some Oxoperoxopolycarboxylato Vanadates (V) And Their Biochemical Significance, Myunghi Lee

Dissertations, Theses, and Masters Projects

No abstract provided.


The Presence Of Acid Polysaccharides In The Mesogloea Of Chrysaora Quinquecirrha, Edward P. Gardner Jan 1975

The Presence Of Acid Polysaccharides In The Mesogloea Of Chrysaora Quinquecirrha, Edward P. Gardner

Dissertations, Theses, and Masters Projects

No abstract provided.


Variations In Major Cations And Certain Heavy Metals In The Serum Of The Blue Crab, Callinectes Sapidus, James Alden Colvocoresses Jan 1973

Variations In Major Cations And Certain Heavy Metals In The Serum Of The Blue Crab, Callinectes Sapidus, James Alden Colvocoresses

Dissertations, Theses, and Masters Projects

No abstract provided.


Effect Of Environmental Salinity On The Amino Acids Of Balanus Improvisus Darwin, Joan L. Faunce Jan 1965

Effect Of Environmental Salinity On The Amino Acids Of Balanus Improvisus Darwin, Joan L. Faunce

Dissertations, Theses, and Masters Projects

No abstract provided.


The Effects Of Environmental Salinity On Free Amino Acids Of Crassostrea Virginica, Maurice Patrick Lynch Jan 1965

The Effects Of Environmental Salinity On Free Amino Acids Of Crassostrea Virginica, Maurice Patrick Lynch

Dissertations, Theses, and Masters Projects

No abstract provided.