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The Development Of Peptide Ligands To Target H69 Rrna, Danielle Nicole Dremann
The Development Of Peptide Ligands To Target H69 Rrna, Danielle Nicole Dremann
Wayne State University Dissertations
ABSTRACT
THE DEVELOPMENT OF PEPTIDE LIGANDS TO TARGET H69
by
DANIELLE NICOLE DREMANN
December 2015
Advisor: Prof. Christine S. Chow
Major: Chemistry (Biochemistry)
Degree: Doctor of Philosophy
In the development of peptide ligands to target H69, SPPS and ESI MS was used to determine if 1) peptides could bind to modified H69 and 2) if increased affinity for the target RNA could be enhanced with modification. An alanine and arginine scan was synthesized and tested for this determination. Selected peptides were then tested using biophysical techniques such as circular dichroism and isothermal titration calorimetry. An assay was also designed to …