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Investigating Saxitoxin Resistance In Softshell Clams (Mya Arenaria): Patterns Of Inheritance And Improvements On Methodology For Tracking And Identification, Scott A. Hamilton
Investigating Saxitoxin Resistance In Softshell Clams (Mya Arenaria): Patterns Of Inheritance And Improvements On Methodology For Tracking And Identification, Scott A. Hamilton
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Attempts to characterize and study the population dynamics of the softshell clam Mya arenaria in relation to a mutation which confers resistance to paralytic shellfish toxins are complicated by a lack of non-lethal genotyping techniques, reliable tagging methods and an understanding of the inheritance patterns of the marker. Presented here, is a straightforward and non-lethal technique for clam genotyping, a new method for the long term tagging of clams, and the offspring genotype frequencies from a number of pair matings between clams of known genotype. Hemolymph extracted from M. arenaria was used directly in a polymerase chain reaction (PCR) to …