Open Access. Powered by Scholars. Published by Universities.®
Physical Sciences and Mathematics Commons™
Open Access. Powered by Scholars. Published by Universities.®
- Institution
- Keyword
-
- Proteins (2)
- And TCPO (1)
- Anions (1)
- Capillary electrophoresis (1)
- Capillary-Channeled Polymer Fibers (1)
-
- Chemiluminescences and Peroxyoxalate (1)
- Cholesterol (1)
- Cholesterol Esterase (1)
- Cholesterol Oxidase (1)
- Citral (1)
- ESI (1)
- Hydrogen Peroxide (1)
- Lemon grass (Cymbopogon citratus) (1)
- MALDI (1)
- Macromolecules (1)
- Polymer Fibers (1)
- Protein Adsorption (1)
- ReversedPhase Chromatography (1)
- SPE (1)
- Stationary Phases (1)
- Volatile oil (1)
Articles 1 - 7 of 7
Full-Text Articles in Physical Sciences and Mathematics
Incorporation Of Capillary-Channeled Polymer (C-Cp) Fibers Into Micropipette Tips For Solid Phase Extraction With Applications In Bioanalysis, Donald Fornea
All Theses
Solid phase extraction (SPE), is a widely used sample preparation method that is currently experiencing a surge in research interest, clever architecture/format development, and stationary phase characterization.1-3 SPE devices commonly exist in syringe, cartridge, disk, and micropipette tip formats.4 Micropipette tip SPE has enhanced the determination of proteins by electrospray ionization (ESI) and matrix assisted laser desorption ionization (MALDI) mass spectrometry (MS) by handling micro-liter volumes of sample and buffer removal/solvent exchange.5-7 Buffers have deleterious effects on ionization taking place in MS sources due to preferential ionization, ion suppression effects, and the formation of adduct species competing for ionization in …
Characterization Of Capillary-Channeled Polymer Fibers As Stationary Phases For High-Performance Liquid Chromatography Of Macromolecules, Dwella Nelson
All Dissertations
Capillary-channeled polymer (C-CP) fibers are being developed and characterized as high-performance liquid chromatography (HPLC) stationary phases for reversed-phase separations of proteins. Conventional porous phases are not well suited for separating large macromolecules due to the slow diffusivity of the molecules, large diffusional distances, and stagnant mobile phase zones within the column. Stationary phases are continually being developed to address the challenges associated with HPLC of proteins and other macromolecules in general. C-CP fiber stationary phases are an alternative to conventional bead technology and offer a variety of chemical and physical advantages. The fibers can be selected for their chemical functionality …
Reversed-Phase Hplc Determination Of Citral In Locally Grown Lemon Grass Harvested At Different Season., Mahmoud Al-Shaer
Reversed-Phase Hplc Determination Of Citral In Locally Grown Lemon Grass Harvested At Different Season., Mahmoud Al-Shaer
Electronic Theses and Dissertations
A simple HPLC procedure for the quantitative determination of citral, the major fragrant component in the lemon grass, has been developed. The procedure involves a C-8 stationary phase using a 90:10 methanol: water pH 5 mobile phase containing 0.25% 1-octanesulfonic acid and an UV detector (set at 233 nm). The lemon grass leaves were harvested fresh at different times of the year and were soaked in methanol for 48 hours without any mechanical assistance to extract the citral and other methanol soluble components. The method showed good reproducibility with relative standard deviation of 2.8% and 10.8% for two different sets …
Determination Of Cholesterol In Foods By Flow Injection Analysis With Perroxyoxalate Chemiluminescence., Abisake Feleke
Determination Of Cholesterol In Foods By Flow Injection Analysis With Perroxyoxalate Chemiluminescence., Abisake Feleke
Electronic Theses and Dissertations
Cholesterol is an important biological molecule with many important functions. However, high serum cholesterol is a health hazard. Thus analysis of cholesterol is important and many analytical techniques have been developed. The objectives of the proposed research are to develop an economical, rapid method for the determination of total cholesterol with good selectivity and enhanced sensitivity. For evaluation of proposed flow injection analysis with peroxyoxalate chemiluminescence (FIA-POCL) method, figures of merit such as accuracy, precision, and linear dynamic range will be assessed. The proposed procedure was then applied to the determination of total cholesterol in foods. The procedure was linear …
Novel Derivatizing Agents For The Determination Of Methylmercury By Gas Chromatography Using Electron Capture Detection, Crystal Marie Irwin
Novel Derivatizing Agents For The Determination Of Methylmercury By Gas Chromatography Using Electron Capture Detection, Crystal Marie Irwin
Dissertations, Theses, and Masters Projects
No abstract provided.
Automated Peak Identification For Time -Of -Flight Mass Spectroscopy, Haijian Chen
Automated Peak Identification For Time -Of -Flight Mass Spectroscopy, Haijian Chen
Dissertations, Theses, and Masters Projects
The high throughput capabilities of protein mass fingerprints measurements have made mass spectrometry one of the standard tools for proteomic research, such as biomarker discovery. However, the analysis of large raw data sets produced by the time-of-flight (TOF) spectrometers creates a bottleneck in the discovery process. One specific challenge is the preprocessing and identification of mass peaks corresponding to important biological molecules. The accuracy of mass assignment is another limitation when comparing mass fingerprints with databases.;We have developed an automated peak picking algorithm based on a maximum likelihood approach that effectively and efficiently detects peaks in a time-of-flight secondary ion …
The Determination Of Probes For The Indirect Detection Of Anions In Capillary Zone Electrophoresis Using Light Emitting Diode Detection, Peter Ashley Balding
The Determination Of Probes For The Indirect Detection Of Anions In Capillary Zone Electrophoresis Using Light Emitting Diode Detection, Peter Ashley Balding
Theses : Honours
This study investigated the potential of a number of compounds to act as probes for the indirect detection of inorganic and organic anions separated by capillary zone electrophoresis and using light emitting diodes (LED) as the light source. Desirable characteristics of the probe anion included: high molar absorptivity, moderate to high electrophoretic mobility, and having a wavelength of maximum absorbance near the maximum emission wavelength of a LED. A preliminary study led to the evaluation of eleven possible compounds. Two of these, p-nitrophenol and 4-hydroxy-3,5-dinitrobenzoic acid were selected for further study as they fulfilled the criteria previously mentioned. The optimised …