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Physical Sciences and Mathematics Commons

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Analytical Chemistry

Western University

2015

Bio-oil

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A Study Of Volatile Organic Compounds From Transgenic Arabidopsis Thaliana And Solanum Lycopersicum Plants And Analytical Characterization Of Pyrolysis Bio-Oil, Luis A. Caceres Feb 2015

A Study Of Volatile Organic Compounds From Transgenic Arabidopsis Thaliana And Solanum Lycopersicum Plants And Analytical Characterization Of Pyrolysis Bio-Oil, Luis A. Caceres

Electronic Thesis and Dissertation Repository

A dynamic headspace method was developed for the non-targeted analysis of volatile organic compounds (VOC) in Arabidopsis and Micro-Tom plants. The method allowed to determine differences from wild type (WT) and transformed plants overexpressing the carotene cleavage dioxygenase 1 (CCD1) genes. In the Arabidopsis study it was revealed the presence of unsaturated hydrocarbons, aromatic derivatives, alcohols, and terpenes. Among extracted volatiles, the apocarotenoid compound β-ionone was also detected, four-fold higher in the transgenic plants than the WT. There were not significant differences in the VOCs between the HS of Micro-Tom tomato plants overexpressing the CCD1 genes and WT. …