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Protein Affinity Extraction Of Prostate Specific Antigen (Psa) Using Submicron Spheres, Yao Wang
Protein Affinity Extraction Of Prostate Specific Antigen (Psa) Using Submicron Spheres, Yao Wang
Open Access Theses
PSA has been used as a biomarker for prostate cancer for a long time. To characterize different glycoforms of PSA using techniques like UHPLC and cIEF, concentration and purification of PSA from complex samples becomes necessary. Our group has developed submicron silica spheres based affinity beads to extract PSA through immunoprecipitation. This study focuses on measuring the bead performance when antibody type/amount was varied and PSA concentration was lowered for clinical use. To better satisfy the requirement of large-scale extraction, several attempts were made to increase the bead capacity. The results will be presented and future directions of the project …