Physical Sciences and Mathematics Commons^™

Development Of Chromatography And Mass Spectrometry Methods For Enzyme Kinetics And Inhibition In Glycolysis And The Methylerythritol Phosphate (Mep) Pathways, Allison Carr

Graduate Research Theses & Dissertations

This dissertation focuses on method development for enzymatic activity and inhibition assays for use in screening potential antibacterial compounds. Infection by antibiotic-resistant pathogens in on the rise globally and several enzymes, including the those in the methylerythritol phosphate (MEP) pathway and fructose bisphosphate aldolase, as strong targets for novel pharmaceuticals because they are not present in humans. Several assays exist for the MEP pathway and aldolase, but there is always work to be done to improve sensitivity, increase throughput, and lower cost. This dissertation describes the development of several methods – hydrophilic interaction chromatography (HILIC), liquid chromatography-mass spectrometry (LC-MS), and …