Physical Sciences and Mathematics Commons^™

Quantitative Analysis Of Acetylsalicylic Acid By Q-Nmr (Quantitative-Nuclear Magnetic Resonance Spectroscopy), Charles Flemming Jr.

All Student Theses

Quantitative analysis is an important step during pharmaceutical drug development. Currently, the pharmaceutical industry uses high performance liquid chromatography (HPLC) for quantitative analysis of organic compounds as the preferred technique. The proton nuclear magnetic resonance (NMR) technique has an inherent ability to quantify a given analyte and may be used to quantify organic compounds. Quantitative nuclear magnetic resonance spectroscopy (q-NMR) of acetylsalicylic acid (ASA) was conducted using tert-butyl alcohol (tert-butanol) as the internal standard and deuterated chloroform (CDCl₃) as the solvent. Preparations of ASA solutions in low and high concentration ranges, 1.0 mM – 10.1 …

Analysis Of An Ion Suppression Device Coupled With An Ion Chromatography System, Sam John

All Capstone Projects

A priority of chromatographers is to eliminate extraneous and errant noise that can interfere with any experiment. The goal is to focus on what is being analyzed, and in the case of detectors, modifying the instrument being utilized is the method of choice. One way to do that in ion chromatography (IC) is through the use of a suppressor.

Though not all instruments utilized in IC are equipped with suppressors, it is ideal to help reduce the ions already known to be present in the eluent. The premise of this study is simple in nature, but important in its application. …

Folate-Peg-Histidine For Use In Detection, Capture, And Analysis Of Fr+ Cells, Sean Dwyer

All Capstone Projects

Folate receptor alpha is a membrane-bound protein displaying high affinity for folic acid. This receptor serves as a receptor-mediated transport system of folic acid into cancer and cells associated with inflammation (e.g., macrophages). Folate is a basic component of cell metabolism in both the synthesis of DNA and proteins, which is hypothesized to be necessary for maintaining adequate processes during increased metabolic demand. Interestingly, most normal cells in the body have little or no high affinity folate receptor alpha. Based on these attributes, folate based drug delivery, imaging systems, and diagnostic systems are in several stages of development worldwide. To …

Feasibility Study Of Waste Isolation And Characterization By Instrumental Methods, Janith A. Wiehe-Wazio

All Capstone Projects

The original focus of this project was to develop an in house procedure for the recovery of commonly used science laboratory solvents consisting of Acetone, Ethyl (Ethanol) Alcohol, Hexane, Methanol and Hydrochloric Acid from everyday laboratory waste. However, the projects scope shifted a little further back to basics when it was discovered that Laboratory Waste Safety and Accounting practices were insufficient. It was assumed that identification of waste products was being recorded accurately. It became apparent with the very first waste bottle analysis that chemicals recorded on the standardized waste log sheets were not always what was in the waste …

Tandem Mass Spectrometric Analysis Of Phosphoamino Acid-Metal Ion Complexes, Urmi Patel

All Capstone Projects

The complex of phosphoamino acid with metal ions was prepared by mixing both solutions and analyzed using ESI MS/MS technique. In this study, O-phospho-L-serine and O-phospho-L-tyrosine were used as phosphoamino acids and single charged metal ions such as Li+, Na+, K+, Rb+, Cs+ and Ag+ were used to study the dissociation patterns when combined with phosphoamino acid. The complexes were successfully fragmented in mass spectra using ESI MS/MS technique and the fragmentation products were studied and the dissociation patterns were proposed.