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Linkage Analysis And Compositional Studies Of Β-Glucan From Saccharomyces Cerevisiae And Compositional Studies Of Mannan From Candida Albicans, Clara Arthur
Electronic Theses and Dissertations
The efficacy of a novel carbohydrate extraction procedure was investigated with methylation analysis and alditol acetate method by Gas Chromatography-Mass Spectrometry. A published extraction procedure for β-glucans was compared to one developed in house. Both procedures gave a dominant glucose peak in the Gas chromatogram indicative of successful β-glucan isolation. Further linkage studies showed four linkage positions for β-glucans isolated with the published method; terminal, 1,3-linkage, 1,6-linkage and 1,3,6-linkage, while β-glucans isolated using the new method showed six linkage positions; terminal, 1,3-linkage, 1,6-linkage, 1,4-linkage, 1,2,3-linkage and 1,3,6-linkage. Diminishing β-glucan linkage peaks in the chromatogram for the published method indicated structure …
Optimized Acid/Base Extraction And Structural Characterization Of Β-Glucan From Saccharomyces Cerevisiae, Shardrack O. Asare
Optimized Acid/Base Extraction And Structural Characterization Of Β-Glucan From Saccharomyces Cerevisiae, Shardrack O. Asare
Electronic Theses and Dissertations
β-glucan is a major component of the fungal cell wall consisting of (1→3)-β linked glucose polymers with (1→6)-β linked side chains. The published classical isolation procedure of β-glucan from Saccharomyces cerevisiae is expensive and time-consuming. Thus, the aim of this research was to develop an effective procedure for the extraction of glucans. We have developed a new method for glucan extraction that will be cost effective and will maintain the native structure of the glucan. The method that we developed is 80% faster and utilizes 1/3 of the reagents compared to the published classical method. Further, the method …